Glycopolymers Mimicking GM1 Gangliosides: Cooperativity of Galactose and Neuraminic Acid for Cholera Toxin Recognition

Yuhei Terada, Yu Hoshino, Yoshiko Miura

研究成果: ジャーナルへの寄稿記事

抄録

Glycopolymers mimicking GM1 gangliosides were synthesized by incorporating multiple types of carbohydrates into the polymer backbone. The glycopolymers were immobilized onto gold surfaces, and the interactions with the cholera toxin B subunit (CTB) were analyzed using surface plasmon resonance imaging. The glycopolymer containing both galactose and neuraminic acid showed enhanced recognition of CTB. The interaction was enhanced mainly because of an improvement in the dissociation process by the binding of the neuraminic acid group in the GM1 binding pocket. This cooperativity of galactose and neuraminic acid was achieved by incorporation into the same flexible polymer backbone, and the importance of the close placement of galactose and neuraminic acid groups was revealed. These results will be valuable in medical fields and also for the development of biofunctional materials.

元の言語英語
ページ(範囲)1021-1027
ページ数7
ジャーナルChemistry - An Asian Journal
14
発行部数7
DOI
出版物ステータス出版済み - 4 1 2019

Fingerprint

Neuraminic Acids
G(M1) Ganglioside
Cholera Toxin
Galactose
Polymers
Surface Plasmon Resonance
Surface plasmon resonance
Gold
Carbohydrates
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry

これを引用

Glycopolymers Mimicking GM1 Gangliosides : Cooperativity of Galactose and Neuraminic Acid for Cholera Toxin Recognition. / Terada, Yuhei; Hoshino, Yu; Miura, Yoshiko.

:: Chemistry - An Asian Journal, 巻 14, 番号 7, 01.04.2019, p. 1021-1027.

研究成果: ジャーナルへの寄稿記事

@article{4e3b88e388a24a168422560930b660f4,
title = "Glycopolymers Mimicking GM1 Gangliosides: Cooperativity of Galactose and Neuraminic Acid for Cholera Toxin Recognition",
abstract = "Glycopolymers mimicking GM1 gangliosides were synthesized by incorporating multiple types of carbohydrates into the polymer backbone. The glycopolymers were immobilized onto gold surfaces, and the interactions with the cholera toxin B subunit (CTB) were analyzed using surface plasmon resonance imaging. The glycopolymer containing both galactose and neuraminic acid showed enhanced recognition of CTB. The interaction was enhanced mainly because of an improvement in the dissociation process by the binding of the neuraminic acid group in the GM1 binding pocket. This cooperativity of galactose and neuraminic acid was achieved by incorporation into the same flexible polymer backbone, and the importance of the close placement of galactose and neuraminic acid groups was revealed. These results will be valuable in medical fields and also for the development of biofunctional materials.",
author = "Yuhei Terada and Yu Hoshino and Yoshiko Miura",
year = "2019",
month = "4",
day = "1",
doi = "10.1002/asia.201900053",
language = "English",
volume = "14",
pages = "1021--1027",
journal = "Chemistry - An Asian Journal",
issn = "1861-4728",
publisher = "John Wiley and Sons Ltd",
number = "7",

}

TY - JOUR

T1 - Glycopolymers Mimicking GM1 Gangliosides

T2 - Cooperativity of Galactose and Neuraminic Acid for Cholera Toxin Recognition

AU - Terada, Yuhei

AU - Hoshino, Yu

AU - Miura, Yoshiko

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Glycopolymers mimicking GM1 gangliosides were synthesized by incorporating multiple types of carbohydrates into the polymer backbone. The glycopolymers were immobilized onto gold surfaces, and the interactions with the cholera toxin B subunit (CTB) were analyzed using surface plasmon resonance imaging. The glycopolymer containing both galactose and neuraminic acid showed enhanced recognition of CTB. The interaction was enhanced mainly because of an improvement in the dissociation process by the binding of the neuraminic acid group in the GM1 binding pocket. This cooperativity of galactose and neuraminic acid was achieved by incorporation into the same flexible polymer backbone, and the importance of the close placement of galactose and neuraminic acid groups was revealed. These results will be valuable in medical fields and also for the development of biofunctional materials.

AB - Glycopolymers mimicking GM1 gangliosides were synthesized by incorporating multiple types of carbohydrates into the polymer backbone. The glycopolymers were immobilized onto gold surfaces, and the interactions with the cholera toxin B subunit (CTB) were analyzed using surface plasmon resonance imaging. The glycopolymer containing both galactose and neuraminic acid showed enhanced recognition of CTB. The interaction was enhanced mainly because of an improvement in the dissociation process by the binding of the neuraminic acid group in the GM1 binding pocket. This cooperativity of galactose and neuraminic acid was achieved by incorporation into the same flexible polymer backbone, and the importance of the close placement of galactose and neuraminic acid groups was revealed. These results will be valuable in medical fields and also for the development of biofunctional materials.

UR - http://www.scopus.com/inward/record.url?scp=85062787877&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062787877&partnerID=8YFLogxK

U2 - 10.1002/asia.201900053

DO - 10.1002/asia.201900053

M3 - Article

VL - 14

SP - 1021

EP - 1027

JO - Chemistry - An Asian Journal

JF - Chemistry - An Asian Journal

SN - 1861-4728

IS - 7

ER -