GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans

Daisuke Murata, Kazuko H. Nomura, Katsufumi Dejima, Souhei Mizuguchi, Nana Kawasaki, Yukari Matsuishi-Nakajima, Satsuki Ito, Keiko Gengyo-Ando, Eriko Kage-Nakadai, Shohei Mitani, Kazuya Nomura

研究成果: ジャーナルへの寄稿記事

20 引用 (Scopus)

抄録

Glycosylphosphatidylinositol (GPI)-anchor attachment is one of the most common posttranslational protein modifications. Using the nematode Caenorhabditis elegans, we determined that GPI-anchored proteins are present in germline cells and distal tip cells, which are essential for the maintenance of the germline stem cell niche. We identified 24 C. elegans genes involved in GPI-anchor synthesis. Inhibition of various steps of GPI-anchor synthesis by RNA interference or gene knockout resulted in abnormal development of oocytes and early embryos, and both lethal and sterile phenotypes were observed. The piga-1 gene (orthologue of human PIGA) codes for the catalytic subunit of the phosphatidylinositol N-acetylglucosaminyltransferase complex, which catalyzes the first step of GPI-anchor synthesis. We isolated piga-1 - knockout worms and found that GPI-anchor synthesis is indispensable for the maintenance of mitotic germline cell number. The knockout worms displayed 100% lethality, with decreased mitotic germline cells and abnormal eggshell formation. Using cell-specific rescue of the null allele, we showed that expression of piga-1 in somatic gonads and/or in germline is sufficient for normal embryonic development and the maintenance of the germline mitotic cells. These results clearly demonstrate that GPI-anchor synthesis is indispensable for germline formation and for normal development of oocytes and eggs.

元の言語英語
ページ(範囲)982-995
ページ数14
ジャーナルMolecular biology of the cell
23
発行部数6
DOI
出版物ステータス出版済み - 3 15 2012

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Glycosylphosphatidylinositols
Caenorhabditis elegans
Maintenance
Oocytes
Egg Shell
Stem Cell Niche
Gene Knockout Techniques
Gonads
Post Translational Protein Processing
Phosphatidylinositols
RNA Interference
Eggs
Genes
Embryonic Development
Catalytic Domain
Proteins
Embryonic Structures
Cell Count
Alleles
Phenotype

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

これを引用

Murata, D., Nomura, K. H., Dejima, K., Mizuguchi, S., Kawasaki, N., Matsuishi-Nakajima, Y., ... Nomura, K. (2012). GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans. Molecular biology of the cell, 23(6), 982-995. https://doi.org/10.1091/mbc.E10-10-0855

GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans. / Murata, Daisuke; Nomura, Kazuko H.; Dejima, Katsufumi; Mizuguchi, Souhei; Kawasaki, Nana; Matsuishi-Nakajima, Yukari; Ito, Satsuki; Gengyo-Ando, Keiko; Kage-Nakadai, Eriko; Mitani, Shohei; Nomura, Kazuya.

:: Molecular biology of the cell, 巻 23, 番号 6, 15.03.2012, p. 982-995.

研究成果: ジャーナルへの寄稿記事

Murata, D, Nomura, KH, Dejima, K, Mizuguchi, S, Kawasaki, N, Matsuishi-Nakajima, Y, Ito, S, Gengyo-Ando, K, Kage-Nakadai, E, Mitani, S & Nomura, K 2012, 'GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans', Molecular biology of the cell, 巻. 23, 番号 6, pp. 982-995. https://doi.org/10.1091/mbc.E10-10-0855
Murata D, Nomura KH, Dejima K, Mizuguchi S, Kawasaki N, Matsuishi-Nakajima Y その他. GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans. Molecular biology of the cell. 2012 3 15;23(6):982-995. https://doi.org/10.1091/mbc.E10-10-0855
Murata, Daisuke ; Nomura, Kazuko H. ; Dejima, Katsufumi ; Mizuguchi, Souhei ; Kawasaki, Nana ; Matsuishi-Nakajima, Yukari ; Ito, Satsuki ; Gengyo-Ando, Keiko ; Kage-Nakadai, Eriko ; Mitani, Shohei ; Nomura, Kazuya. / GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans. :: Molecular biology of the cell. 2012 ; 巻 23, 番号 6. pp. 982-995.
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