Growth stimulating activity of heat shock protein 90α to lymphoid cell lines in serum-free medium

Toshihiro Kuroita, Hirofumi Tachibana, Hideya Ohashi, Sanetaka Shirahata, Hiroki Murakami

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

A growth stimulating factor was purified from the culture supernatant of human-human hybridoma SH-76 cells in serum-free RPMI 1640 medium by the serial use of DEAE anion and heparin affinity chromatographies. The factor was a 85 kDa protein on SDS-polyacrylamide gel electrophoresis. N-terminal amino acid sequence (PEETQTQDQPME) of the protein was coincident with that of heat shock protein 90α (HSP90α). The protein reacted with anti-HSP90 monoclonal antibody. These results suggest that the protein was a member of HSP90α family after taking other circumstantial evidence into account. The protein stimulated the growth of some lymphoid cell lines such as human B-lymphoblastoid cell line HO-323-3 hybridomas derived from HO-323, and several other lymphoid cell lines. There were several lymphoid cell lines which did not respond to the protein. Growth stimulating activity of the protein was heat-unstable and significantly decreased at above 60°C. These are the first data that describe growth-stimulating activity of heat shock protein 90α.

元の言語英語
ページ(範囲)109-117
ページ数9
ジャーナルCytotechnology
8
発行部数2
DOI
出版物ステータス出版済み - 6 1 1992

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HSP90 Heat-Shock Proteins
Serum-Free Culture Media
Cells
Lymphocytes
Proteins
Cell Line
Growth
Hybridomas
Affinity chromatography
Hot Temperature
Electrophoresis
Affinity Chromatography
Anions
Heparin
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
Intercellular Signaling Peptides and Proteins
Monoclonal antibodies
Monoclonal Antibodies
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

これを引用

Growth stimulating activity of heat shock protein 90α to lymphoid cell lines in serum-free medium. / Kuroita, Toshihiro; Tachibana, Hirofumi; Ohashi, Hideya; Shirahata, Sanetaka; Murakami, Hiroki.

:: Cytotechnology, 巻 8, 番号 2, 01.06.1992, p. 109-117.

研究成果: ジャーナルへの寄稿記事

Kuroita, Toshihiro ; Tachibana, Hirofumi ; Ohashi, Hideya ; Shirahata, Sanetaka ; Murakami, Hiroki. / Growth stimulating activity of heat shock protein 90α to lymphoid cell lines in serum-free medium. :: Cytotechnology. 1992 ; 巻 8, 番号 2. pp. 109-117.
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