Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure

Sumio Iwai, Sho Ogata, Naotaka Yamada, Michio Onjo, Kintake Sonoike, Ken ichiro Shimazaki

研究成果: ジャーナルへの寄稿記事

抄録

Reactive oxygen species (ROS) are ubiquitous signaling molecules involved in diverse physiological processes, including stomatal closure. Photosynthetic electron transport (PET) is the main source of ROS generation in plants, but whether it functions in guard cell signaling remains unclear. Here, we assessed whether PET functions in abscisic acid (ABA) signaling in guard cells. ABA-elicited ROS were localized to guard cell chloroplasts in Arabidopsis thaliana, Commelina benghalensis, and Vicia faba in the light and abolished by the PET inhibitors 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea and 2, 5-dibromo-3-methyl-6-isopropyl-p-benzoquinone. These inhibitors reduced ABA-induced stomatal closure in all three species, as well as in the NADPH oxidase-lacking mutant atrboh D/F. However, an NADPH oxidase inhibitor did not fully eliminate ABA-induced ROS in the chloroplasts, and ABA-induced ROS were still observed in the guard cell chloroplasts of atrboh D/F. This study demonstrates that ROS generated through PET act as signaling molecules in ABA-induced stomatal closure and that this occurs in concert with ROS derived through NADPH oxidase.

元の言語英語
記事番号e00137
ジャーナルPlant Direct
3
発行部数5
DOI
出版物ステータス出版済み - 5 1 2019

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Abscisic Acid
abscisic acid
Photosynthesis
guard cells
reactive oxygen species
Reactive Oxygen Species
photosynthesis
Electron Transport
electron transfer
NADPH Oxidase
Chloroplasts
chloroplast
inhibitor
electron
chloroplasts
Dibromothymoquinone
Commelina
Commelina benghalensis
Diuron
Physiological Phenomena

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Plant Science

これを引用

Iwai, S., Ogata, S., Yamada, N., Onjo, M., Sonoike, K., & Shimazaki, K. I. (2019). Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure. Plant Direct, 3(5), [e00137]. https://doi.org/10.1002/pld3.137

Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure. / Iwai, Sumio; Ogata, Sho; Yamada, Naotaka; Onjo, Michio; Sonoike, Kintake; Shimazaki, Ken ichiro.

:: Plant Direct, 巻 3, 番号 5, e00137, 01.05.2019.

研究成果: ジャーナルへの寄稿記事

Iwai, S, Ogata, S, Yamada, N, Onjo, M, Sonoike, K & Shimazaki, KI 2019, 'Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure', Plant Direct, 巻. 3, 番号 5, e00137. https://doi.org/10.1002/pld3.137
Iwai, Sumio ; Ogata, Sho ; Yamada, Naotaka ; Onjo, Michio ; Sonoike, Kintake ; Shimazaki, Ken ichiro. / Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure. :: Plant Direct. 2019 ; 巻 3, 番号 5.
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