TY - JOUR
T1 - H-, K-, and N-ras gene mutation in atypical fibroxanthoma and malignant fibrous histiocytoma
AU - Sakamoto, Akio
AU - Oda, Yoshinao
AU - Itakura, Eijun
AU - Oshiro, Yumi
AU - Tamiya, Sadafumi
AU - Honda, Yumi
AU - Ishihara, Akira
AU - Iwamoto, Yukihide
AU - Tsuneyoshi, Masazumi
N1 - Funding Information:
Supported by a grant-in-aid for cancer research from the Fukuoka Cancer Society, Fukuoka, Japan; and a grant-in-aid for general scientific research from the Ministry of Education, Science and Culture (09470052 and 12670167), Tokyo, Japan.
PY - 2001
Y1 - 2001
N2 - Atypical fibroxanthoma (AFX) which is histologically similar to malignant fibrous histiocytoma (MFH), occurs in the sun-exposed skin. The presence of mutations at codons 12 and 13 of the H- and K-ras genes and in exons 1 and 2, which include codons 12, 13, and 61, of the N-ras gene was studied in 8 cases of AFX and 8 cases of storiform-pleomorphic-type MFH using polymerase chain reaction (PCR)-restriction fragment length polymorphism and PCR-single-conformation polymorphism. Two of the 8 cases of MFH showed ras mutations in the H-ras gene at codon 12 (GGC-AGC) and in the K-ras gene at codon 13 (GGC-GAC). H- and K-ras gene mutations were not seen in any of the cases of AFX (0 of 8). N-ras gene mutation was not detected in either the AFX (0 of 8) or MFH (0 of 8) cases. In conclusion, although the number of cases in this study was small, H-and K-ras genes were present in some of the MFH cases and accordingly may play an important role in the pathogenesis of MFH. In addition, the finding that H-, K-, and N-ras gene mutations are not present in AFX may indicate why AFX has a more favorable behavior than MFH.
AB - Atypical fibroxanthoma (AFX) which is histologically similar to malignant fibrous histiocytoma (MFH), occurs in the sun-exposed skin. The presence of mutations at codons 12 and 13 of the H- and K-ras genes and in exons 1 and 2, which include codons 12, 13, and 61, of the N-ras gene was studied in 8 cases of AFX and 8 cases of storiform-pleomorphic-type MFH using polymerase chain reaction (PCR)-restriction fragment length polymorphism and PCR-single-conformation polymorphism. Two of the 8 cases of MFH showed ras mutations in the H-ras gene at codon 12 (GGC-AGC) and in the K-ras gene at codon 13 (GGC-GAC). H- and K-ras gene mutations were not seen in any of the cases of AFX (0 of 8). N-ras gene mutation was not detected in either the AFX (0 of 8) or MFH (0 of 8) cases. In conclusion, although the number of cases in this study was small, H-and K-ras genes were present in some of the MFH cases and accordingly may play an important role in the pathogenesis of MFH. In addition, the finding that H-, K-, and N-ras gene mutations are not present in AFX may indicate why AFX has a more favorable behavior than MFH.
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U2 - 10.1053/hupa.2001.28953
DO - 10.1053/hupa.2001.28953
M3 - Article
C2 - 11727262
AN - SCOPUS:0035191597
VL - 32
SP - 1225
EP - 1231
JO - Human Pathology
JF - Human Pathology
SN - 0046-8177
IS - 11
ER -