Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision

Tapas Chakraborty, Sipra Mohapatra, Megumi Tobayama, Kayoko Ohta, Yong Woon Ryu, Yukinori Kazeto, Kohei Ohta, Linyan Zhou, Yoshitaka Nagahama, Takahiro Matsubara

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Environmental stressors, gonadal degenerative diseases and tumour development can significantly alter the oocyte physiology, and species fertility and fitness. To expand the molecular understanding about oocyte degradation, we isolated several spliced variants of Japanese anchovy hatching enzymes (AcHEs; ovastacin homologue) 1 and 2, and analysed their potential in oocyte sustenance. Particularly, AcHE1b, an ovary-specific, steroid-regulated, methylation-dependent, stress-responsive isoform, was neofunctionalized to regulate autophagic oocyte degeneration. AcHE1a and 2 triggered apoptotic degeneration in vitellogenic and mature oocytes, respectively. Progesterone, starvation, and high temperature elevated the total degenerating oocyte population and AcHE1b transcription by hyper-demethylation. Overexpression, knockdown and intracellular zinc ion chelation study confirmed the functional significance of AcHE1b in autophagy induction, possibly to mitigate the stress effects in fish, via ion-homeostasis. Our finding chronicles the importance of AcHEs in stress-influenced apoptosis/autophagy cell fate decision and may prove significant in reproductive failure assessments, gonadal health maintenance and ovarian degenerative disease therapy.

元の言語英語
記事番号3183
ジャーナルScientific reports
7
発行部数1
DOI
出版物ステータス出版済み - 12 1 2017

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envelysin
Autophagy
Oocytes
Apoptosis
Ovarian Diseases
Ions
Pain
Starvation
Methylation
Progesterone
Fertility
Zinc
Ovary
Protein Isoforms
Fishes
Homeostasis
Steroids

All Science Journal Classification (ASJC) codes

  • General

これを引用

Chakraborty, T., Mohapatra, S., Tobayama, M., Ohta, K., Ryu, Y. W., Kazeto, Y., ... Matsubara, T. (2017). Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision. Scientific reports, 7(1), [3183]. https://doi.org/10.1038/s41598-017-03314-7

Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision. / Chakraborty, Tapas; Mohapatra, Sipra; Tobayama, Megumi; Ohta, Kayoko; Ryu, Yong Woon; Kazeto, Yukinori; Ohta, Kohei; Zhou, Linyan; Nagahama, Yoshitaka; Matsubara, Takahiro.

:: Scientific reports, 巻 7, 番号 1, 3183, 01.12.2017.

研究成果: ジャーナルへの寄稿記事

Chakraborty, T, Mohapatra, S, Tobayama, M, Ohta, K, Ryu, YW, Kazeto, Y, Ohta, K, Zhou, L, Nagahama, Y & Matsubara, T 2017, 'Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision', Scientific reports, 巻. 7, 番号 1, 3183. https://doi.org/10.1038/s41598-017-03314-7
Chakraborty, Tapas ; Mohapatra, Sipra ; Tobayama, Megumi ; Ohta, Kayoko ; Ryu, Yong Woon ; Kazeto, Yukinori ; Ohta, Kohei ; Zhou, Linyan ; Nagahama, Yoshitaka ; Matsubara, Takahiro. / Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision. :: Scientific reports. 2017 ; 巻 7, 番号 1.
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