Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe

Yasuko Fujita, Hideki Tohda, Yuko Giga-Hama, Kaoru Takegawa

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

A new, heat shock-inducible expression system based on an endogenous hsp16+ promoter was developed for use in the fission yeast Schizosaccharomyces pombe. Analysis of GFP expression profiles indicated that a 1.2-kb segment of the hsp16+ promoter region was sufficient to drive expression of heterologous protein. The hsp16+ promoter was found to be activated not only by heat shock but also by other stresses including cadmium, ethanol, and oxidative stress. Two expression vectors, pHIL and pHIU, were constructed using the 1.2-kb hsp16+ promoter for inducible gene expression in Sch. pombe. This new expression system utilizes a simple induction protocol and promises to be a useful tool for analyzing gene expression in Sch. pombe.

元の言語英語
ページ(範囲)883-887
ページ数5
ジャーナルFEMS Yeast Research
6
発行部数6
DOI
出版物ステータス出版済み - 9 1 2006
外部発表Yes

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Schizosaccharomyces
Shock
Hot Temperature
Gene Expression
Cadmium
Genetic Promoter Regions
Oxidative Stress
Ethanol
Proteins

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Infectious Diseases

これを引用

Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe. / Fujita, Yasuko; Tohda, Hideki; Giga-Hama, Yuko; Takegawa, Kaoru.

:: FEMS Yeast Research, 巻 6, 番号 6, 01.09.2006, p. 883-887.

研究成果: ジャーナルへの寄稿記事

Fujita, Yasuko ; Tohda, Hideki ; Giga-Hama, Yuko ; Takegawa, Kaoru. / Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe. :: FEMS Yeast Research. 2006 ; 巻 6, 番号 6. pp. 883-887.
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