Heat treatment of retinal pigment epithelium induces production of elastic lamina components and antiangiogenic activity

Eiichi Sekiyama, Magali Saint-Geniez, Kazuhito Yoneda, Toshio Hisatomi, Shintaro Nakao, Tony E. Walshe, Kazuichi Maruyama, Ali Hafezi-Moghadam, Joan W. Miller, Shigeru Kinoshita, Patricia A. D'Amore

研究成果: Contribution to journalArticle査読

9 被引用数 (Scopus)

抄録

Age-related macular degeneration (AMD) is the leading cause of blindness in the Western world. In advanced AMD, new vessels from choriocapillaris (CC) invade through the Bruch's membrane (BrM) into the retina, forming choroidal neovascularization (CNV). BrM, an elastic lamina that is located between the retinal pigment epithelium (RPE) and CC, is thought to act as a physical and functional barrier against CNV. The BrM of patients with early AMD are characterized by decreased levels of antiangiogenic factors, including endostatin, thrombospondin-1 (TSP-1), and pigment epithelium-derived factor (PEDF), as well as by degeneration of the elastic layer. Motivated by a previous report that heat increases elastin expression in human skin, we examined the effect of heat on human ARPE-19 cell production of BrM components. Heat treatment stimulated the production of BrM components, including TSP-1, PEDF, and tropoelastin in vitro and increased the antiangiogenic activity of RPE measured in amouse corneal pocket assay. The effect of heat on experimental CNV was investigated by pretreating the retina with heat via infrared diode laser prior to the induction of CNV. Heat treatment blocked the development of experimental CNV in vivo. These findings suggest that heat treatment may restore BrM integrity and barrier function against new vessel growth.

本文言語英語
ページ(範囲)567-575
ページ数9
ジャーナルFASEB Journal
26
2
DOI
出版ステータス出版済み - 2 2012

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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