Hedgehog Gli3 activator signal augments tumorigenicity of colorectal cancer via upregulation of adherence-related genes

Hironori Iwasaki, kenji nakano, Kentaro Shinkai, Yumi Kunisawa, Minako Hirahashi, Yoshinao Oda, Hideya Ohnishi, Mitsuo Katano

研究成果: ジャーナルへの寄稿記事

19 引用 (Scopus)

抄録

Hedgehog signal is re-activated in several cancers. In this study, we examined the role of Gli3 on malignant phenotype of tumorigenicity for colorectal cancer and its relationship with p53, WNT and ERK/AKT signals. Gli3 expression was detected in HT29 and SW480 (p53-mutant) cells, but not in DLD-1 (p53-mutant) or HCT116 (p53-wild type) cells by reverse transcription-polymerase chain reaction and immunocytochemistry. Full-length Gli3 transfection increased anchor-independent growth for all cells regardless of p53 status, with upregulation of adhesion-related genes. Exogenous Sonic-Hedgehog increased activator-type of Gli3 and colony formation in Gli3-positive HT29 and SW480 cells. After implantation of Gli3-FL or mock-transfectant DLD-1 cells into SCID mice, tumor formation was highly observed in only Gli3-FL-transfectant group. In clinical specimens, Gli3 expression was detected in subsets of colorectal cancer and related with poorly-differentiated histological type, while Sonic-Hedgehog was present with high incidence. In conclusion, activator Gli3 signal augments tumorigenicity of colorectal cancer irrespective of p53 status.

元の言語英語
ページ(範囲)328-336
ページ数9
ジャーナルCancer Science
104
発行部数3
DOI
出版物ステータス出版済み - 3 1 2013

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Hedgehogs
Colorectal Neoplasms
Up-Regulation
Genes
HT29 Cells
SCID Mice
Reverse Transcription
Transfection
Neoplasms
Immunohistochemistry
Phenotype
Polymerase Chain Reaction
Incidence
Growth

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Hedgehog Gli3 activator signal augments tumorigenicity of colorectal cancer via upregulation of adherence-related genes. / Iwasaki, Hironori; nakano, kenji; Shinkai, Kentaro; Kunisawa, Yumi; Hirahashi, Minako; Oda, Yoshinao; Ohnishi, Hideya; Katano, Mitsuo.

:: Cancer Science, 巻 104, 番号 3, 01.03.2013, p. 328-336.

研究成果: ジャーナルへの寄稿記事

Iwasaki, Hironori ; nakano, kenji ; Shinkai, Kentaro ; Kunisawa, Yumi ; Hirahashi, Minako ; Oda, Yoshinao ; Ohnishi, Hideya ; Katano, Mitsuo. / Hedgehog Gli3 activator signal augments tumorigenicity of colorectal cancer via upregulation of adherence-related genes. :: Cancer Science. 2013 ; 巻 104, 番号 3. pp. 328-336.
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AU - Oda, Yoshinao

AU - Ohnishi, Hideya

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