Helicase proteins DHX29 and RIG-I cosense cytosolic nucleic acids in the human airway system

Naoshi Sugimoto, Hiroki Mitoma, Taeil Kim, Shino Hanabuchi, Yong Jun Liu

研究成果: ジャーナルへの寄稿記事

16 引用 (Scopus)

抄録

The recognition of cytoplasmic nucleic acid is critical for innate immune responses against microbial infection and is responsible for autoimmunity induced by dead cells. Here, we report the identification of a unique cytosolic nucleic acid cosensor in human airway epithelial cells and fibroblasts: DEAH (Asp-Glu-Ala-His) box poly-peptide 29 (DHX29), a member of the DExD/H (Asp-Glu-x-Asp/His)-box helicase family. Knocking down DHX29 by siRNA attenuated the ability of cells to mount type I IFN and IL-6 in response to cyto-solic nucleic acids and various viruses by blocking the activation of interferon regulatory factor 3 and NF-κB-p65. The cytosolic nucleic acid sensing by DHX29 in human epithelial cells and fibroblasts is independent of stimulator of interferon genes but is dependent on retinoic acid-inducible gene 1 (RIG-I) and mitochondrial antiviral signaling protein (MAVS). DHX29 binds directly to nucleic acids and interacts with RIG-I and MAVS through its helicase 1 domain, activating the RIG-I-MAVS-dependent cytosolic nucleic acid response. These results suggest that DHX29 is a cytosolic nucleic acid cosensor that triggers RIG-I/MAVS-dependent signaling pathways. This study will have important implications in drug and vaccine design for control of viral infections and viral-induced pathology in the airway.

元の言語英語
ページ(範囲)7747-7752
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
111
発行部数21
DOI
出版物ステータス出版済み - 5 27 2014

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Nucleic Acids
Antiviral Agents
Proteins
aspartylglutamate
Fibroblasts
Epithelial Cells
Interferon Regulatory Factor-3
Virus Activation
Drug Design
Virus Diseases
Tretinoin
Autoimmunity
Innate Immunity
Interferons
Small Interfering RNA
Genes
Interleukin-6
Vaccines
Pathology
Peptides

All Science Journal Classification (ASJC) codes

  • General

これを引用

Helicase proteins DHX29 and RIG-I cosense cytosolic nucleic acids in the human airway system. / Sugimoto, Naoshi; Mitoma, Hiroki; Kim, Taeil; Hanabuchi, Shino; Liu, Yong Jun.

:: Proceedings of the National Academy of Sciences of the United States of America, 巻 111, 番号 21, 27.05.2014, p. 7747-7752.

研究成果: ジャーナルへの寄稿記事

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