Helicobacter pylori lipopolysaccharide activates Rac1 and transcription of NADPH oxidase Nox1 and its organizer NOXO1 in guinea pig gastric mucosal cells

Tsukasa Kawahara, Motoyuki Kohjima, Yuki Kuwano, Hisano Mino, Shigetada Teshima-Kondo, Ryu Takeya, Shohko Tsunawaki, Akihiro Wada, Hideki Sumimoto, Kazuhito Rokutan

研究成果: ジャーナルへの寄稿学術誌査読

117 被引用数 (Scopus)

抄録

Primary cultures of guinea pig gastric mucosal cells express NADPH oxidase 1 (Nox1), a homolog of gp91phox, and produce superoxide anion (O 2-) at a rate of ∼100 nmol·mg protein -1· h-1 in response to Helicobacter pylori (H. pylori) lipopolysaccharide (LPS) from virulent type I strains. The upregulated O2- production also enhances H. pylori LPS-stimulated tumor necrosis factor-α or cyclooxygenase-2 mRNA expression, which suggests a potential role for Nox1 in the pathogenesis of H. pylori-associated diseases. The H. pylori LPS-stimulated O2- production in cultured gastric mucosal cells was inhibited by actinomycin D as well as cycloheximide, suggesting that the induction is regulated at the transcriptional level. The LPS treatment not only increased the Nox1 mRNA to a greater extent but also induced expression of the message-encoding, Nox-organizing protein 1 (NOXO1), a novel p47phox homolog required for Nox1 activity. In addition, H. pylori LPS activated Rac1; i.e., it converted Rac1 to the GTP-bound state. A phosphoinositide 3-kinase inhibitor, LY-294002, blocked H. pylori LPS-induced Rac1 activation and O2- generation without interfering with the expression of Nox1 and NOXO1 mRNA. O2 - production inhibited by LY-294002 was completely restored by transfection of an adenoviral vector encoding a constitutively active Rac1 but not an inactive Rac1 or a constitutively active Cdc42. These findings indicate that Rac1 plays a crucial role in Nox1 activation. Thus the H. pylori LPS-stimulated O2- production in gastric mucosal cells appears to require two distinct events: 1) transcriptional upregulation of Nox1 and NOXO1 and 2) activation of Rac1.

本文言語英語
ページ(範囲)C450-C457
ジャーナルAmerican Journal of Physiology - Cell Physiology
288
2 57-2
DOI
出版ステータス出版済み - 2月 2005

!!!All Science Journal Classification (ASJC) codes

  • 生理学
  • 細胞生物学

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