Hematopoietic tissue-specific expression of mouse Neil3 for endonuclease VIII-like protein

研究成果: ジャーナルへの寄稿記事

50 引用 (Scopus)

抄録

We cloned cDNA and genomic DNA containing exon 1 of mouse Neil3. Neil3 spans 52.4 kb and consists of 10 exons. Northern blot analysis revealed that Neil3 mRNA was selectively expressed in thymus, spleen and bone marrow. High levels of Neil3 mRNA were also detected in various mouse B cell lines by RT-PCR. Immunofluorescence microscopy using anti-NEIL3 revealed that recombinant mouse NEIL3 is localized in the nuclei. In mouse splenocytes, the level of Neil3 mRNA significantly increased after mitogen stimulation in vitro. We established NEIL3-null mice, which are viable and fertile. We found candidate sequences for NEIL3 orthologues in a DNA database from dog and zebrafish in addition to human and mouse, but not invertebrates. NEIL3 may function exclusively in vertebrates, such as mammals.

元の言語英語
ページ(範囲)763-772
ページ数10
ジャーナルJournal of biochemistry
138
発行部数6
DOI
出版物ステータス出版済み - 12 1 2005

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Deoxyribonuclease (Pyrimidine Dimer)
Tissue
Messenger RNA
Exons
Thymus
Proteins
Mammals
DNA
Mitogens
Microscopic examination
Bone
Complementary DNA
Cells
Nucleic Acid Databases
Zebrafish
Invertebrates
Fluorescence Microscopy
Northern Blotting
Thymus Gland
Vertebrates

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

Hematopoietic tissue-specific expression of mouse Neil3 for endonuclease VIII-like protein. / torisu, kumiko; Tsuchimoto, Daisuke; Ohnishi, Yoshinori; Nakabeppu, Yusaku.

:: Journal of biochemistry, 巻 138, 番号 6, 01.12.2005, p. 763-772.

研究成果: ジャーナルへの寄稿記事

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