Hemolymph proteins during larval development of bombyx mori

Proteins of young larvae

Yutaka Banno, Yutaka Kawaguchi, Hiroshi Doira, Shinji Tochihara

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Hemolymph proteins were analyzed quantitatively and qualitatively throughout the larval development of Bombyx mori. The hemolymph protein concentration decreased by the 4th larval instar, with cyclic increases until molting and decreases after ecdysis. The protein concentration increased rapidly after the 4th molt and reached a maximum value at the end of the 5th instar, with female larvae showing higher concentration than the male ones. This sexual dimorphism was obtained only after day 2 of the 5th instar. The electrophoretic analysis of hemolymph proteins revealed the existence of two groups of major proteins from hatching to the 3rd larval instar. Their content decreased gradually in the 4th instar and they disappeared in the 5th instar. The two groups should be referred to as “Proteins of young larvae -A and -B (PYL-A and PYL-B)”. PYL-A and PYL-B consisted of four and three proteins, respectively. In the late 5th instar, hemolymph proteins consisted of 3 major proteins (SP-2, 30kDa proteins and FL-1 with the latter being minor in the male) and many minor ones. These results clearly indicated that characteristics of the hemolymph at the young larval stage (1st instar to 3rd instar) were very different from those of the 5th instar in terms of the major protein and total protein concentrations.

元の言語英語
ページ(範囲)187-194
ページ数8
ジャーナルJournal of Sericultural Science of Japan
62
発行部数3
DOI
出版物ステータス出版済み - 1993

Fingerprint

Bombyx
Hemolymph
Bombyx mori
larval development
hemolymph
Larva
Proteins
instars
larvae
proteins
Molting
molting
Protein
ecdysis
Sex Characteristics
sexual dimorphism
hatching

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Polymers and Plastics

これを引用

Hemolymph proteins during larval development of bombyx mori : Proteins of young larvae. / Banno, Yutaka; Kawaguchi, Yutaka; Doira, Hiroshi; Tochihara, Shinji.

:: Journal of Sericultural Science of Japan, 巻 62, 番号 3, 1993, p. 187-194.

研究成果: ジャーナルへの寄稿記事

Banno, Yutaka ; Kawaguchi, Yutaka ; Doira, Hiroshi ; Tochihara, Shinji. / Hemolymph proteins during larval development of bombyx mori : Proteins of young larvae. :: Journal of Sericultural Science of Japan. 1993 ; 巻 62, 番号 3. pp. 187-194.
@article{1c549806b00c4da4b93d27c63851b354,
title = "Hemolymph proteins during larval development of bombyx mori: Proteins of young larvae",
abstract = "Hemolymph proteins were analyzed quantitatively and qualitatively throughout the larval development of Bombyx mori. The hemolymph protein concentration decreased by the 4th larval instar, with cyclic increases until molting and decreases after ecdysis. The protein concentration increased rapidly after the 4th molt and reached a maximum value at the end of the 5th instar, with female larvae showing higher concentration than the male ones. This sexual dimorphism was obtained only after day 2 of the 5th instar. The electrophoretic analysis of hemolymph proteins revealed the existence of two groups of major proteins from hatching to the 3rd larval instar. Their content decreased gradually in the 4th instar and they disappeared in the 5th instar. The two groups should be referred to as “Proteins of young larvae -A and -B (PYL-A and PYL-B)”. PYL-A and PYL-B consisted of four and three proteins, respectively. In the late 5th instar, hemolymph proteins consisted of 3 major proteins (SP-2, 30kDa proteins and FL-1 with the latter being minor in the male) and many minor ones. These results clearly indicated that characteristics of the hemolymph at the young larval stage (1st instar to 3rd instar) were very different from those of the 5th instar in terms of the major protein and total protein concentrations.",
author = "Yutaka Banno and Yutaka Kawaguchi and Hiroshi Doira and Shinji Tochihara",
year = "1993",
doi = "10.11416/kontyushigen1930.62.187",
language = "English",
volume = "62",
pages = "187--194",
journal = "Journal of Insect Biotechnology and Sericology",
issn = "1346-8073",
publisher = "Japanese Society of Sericultural Sciences",
number = "3",

}

TY - JOUR

T1 - Hemolymph proteins during larval development of bombyx mori

T2 - Proteins of young larvae

AU - Banno, Yutaka

AU - Kawaguchi, Yutaka

AU - Doira, Hiroshi

AU - Tochihara, Shinji

PY - 1993

Y1 - 1993

N2 - Hemolymph proteins were analyzed quantitatively and qualitatively throughout the larval development of Bombyx mori. The hemolymph protein concentration decreased by the 4th larval instar, with cyclic increases until molting and decreases after ecdysis. The protein concentration increased rapidly after the 4th molt and reached a maximum value at the end of the 5th instar, with female larvae showing higher concentration than the male ones. This sexual dimorphism was obtained only after day 2 of the 5th instar. The electrophoretic analysis of hemolymph proteins revealed the existence of two groups of major proteins from hatching to the 3rd larval instar. Their content decreased gradually in the 4th instar and they disappeared in the 5th instar. The two groups should be referred to as “Proteins of young larvae -A and -B (PYL-A and PYL-B)”. PYL-A and PYL-B consisted of four and three proteins, respectively. In the late 5th instar, hemolymph proteins consisted of 3 major proteins (SP-2, 30kDa proteins and FL-1 with the latter being minor in the male) and many minor ones. These results clearly indicated that characteristics of the hemolymph at the young larval stage (1st instar to 3rd instar) were very different from those of the 5th instar in terms of the major protein and total protein concentrations.

AB - Hemolymph proteins were analyzed quantitatively and qualitatively throughout the larval development of Bombyx mori. The hemolymph protein concentration decreased by the 4th larval instar, with cyclic increases until molting and decreases after ecdysis. The protein concentration increased rapidly after the 4th molt and reached a maximum value at the end of the 5th instar, with female larvae showing higher concentration than the male ones. This sexual dimorphism was obtained only after day 2 of the 5th instar. The electrophoretic analysis of hemolymph proteins revealed the existence of two groups of major proteins from hatching to the 3rd larval instar. Their content decreased gradually in the 4th instar and they disappeared in the 5th instar. The two groups should be referred to as “Proteins of young larvae -A and -B (PYL-A and PYL-B)”. PYL-A and PYL-B consisted of four and three proteins, respectively. In the late 5th instar, hemolymph proteins consisted of 3 major proteins (SP-2, 30kDa proteins and FL-1 with the latter being minor in the male) and many minor ones. These results clearly indicated that characteristics of the hemolymph at the young larval stage (1st instar to 3rd instar) were very different from those of the 5th instar in terms of the major protein and total protein concentrations.

UR - http://www.scopus.com/inward/record.url?scp=0042333937&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042333937&partnerID=8YFLogxK

U2 - 10.11416/kontyushigen1930.62.187

DO - 10.11416/kontyushigen1930.62.187

M3 - Article

VL - 62

SP - 187

EP - 194

JO - Journal of Insect Biotechnology and Sericology

JF - Journal of Insect Biotechnology and Sericology

SN - 1346-8073

IS - 3

ER -