抄録
The cell morphology and expression of differentiated functions of primary rat hepatocytes on a titanium dioxide (TiO2) gel surface were investigated. Polystyrene culture dishes were coated with TiO2 gel by spin-coating an ethanol solution of titanium n-butoxide, hydrolyzing in a humidity chamber and drying with nitrogen gas. The TiO2 gel layer formed on the polystyrene dishes was transparent and robust, and its surface was quite flat. Rat hepatocytes inoculated on the TiO2 gel-coated polystyrene dishes gradually accumulated with increasing culture time, and then spontaneously formed many hepatocyte spheroids at 90 ± 21 μm diameter from about 3 days of culture. The diameter of the spheroids increased during the culture, and was 151 ± 43 μm at 14 days of culture. Ammonia removal and albumin secretion by hepatocytes on the TiO2 gel-coated polystyrene dishes were maintained at a high level for at least 14 days of culture compared with on a type I collagen-coated dish and a non-coated polystyrene dish. These results indicate that TiO2 gel is an adequate material for hepatocyte spheroid formation and long-term culture of spheroids.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 359-364 |
| ページ数 | 6 |
| ジャーナル | Journal of Materials Science: Materials in Medicine |
| 巻 | 17 |
| 発行部数 | 4 |
| DOI | |
| 出版物ステータス | 出版済み - 4 1 2006 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Bioengineering
- Biomaterials
- Biomedical Engineering
これを引用
Hepatocyte spheroid formation on a titanium dioxide gel surface and hepatocyte long-term culture. / Nakazawa, K.; Lee, S. W.; Fukuda, J.; Yang, D. H.; Kunitake, T.
：: Journal of Materials Science: Materials in Medicine, 巻 17, 番号 4, 01.04.2006, p. 359-364.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Hepatocyte spheroid formation on a titanium dioxide gel surface and hepatocyte long-term culture
AU - Nakazawa, K.
AU - Lee, S. W.
AU - Fukuda, J.
AU - Yang, D. H.
AU - Kunitake, T.
PY - 2006/4/1
Y1 - 2006/4/1
N2 - The cell morphology and expression of differentiated functions of primary rat hepatocytes on a titanium dioxide (TiO2) gel surface were investigated. Polystyrene culture dishes were coated with TiO2 gel by spin-coating an ethanol solution of titanium n-butoxide, hydrolyzing in a humidity chamber and drying with nitrogen gas. The TiO2 gel layer formed on the polystyrene dishes was transparent and robust, and its surface was quite flat. Rat hepatocytes inoculated on the TiO2 gel-coated polystyrene dishes gradually accumulated with increasing culture time, and then spontaneously formed many hepatocyte spheroids at 90 ± 21 μm diameter from about 3 days of culture. The diameter of the spheroids increased during the culture, and was 151 ± 43 μm at 14 days of culture. Ammonia removal and albumin secretion by hepatocytes on the TiO2 gel-coated polystyrene dishes were maintained at a high level for at least 14 days of culture compared with on a type I collagen-coated dish and a non-coated polystyrene dish. These results indicate that TiO2 gel is an adequate material for hepatocyte spheroid formation and long-term culture of spheroids.
AB - The cell morphology and expression of differentiated functions of primary rat hepatocytes on a titanium dioxide (TiO2) gel surface were investigated. Polystyrene culture dishes were coated with TiO2 gel by spin-coating an ethanol solution of titanium n-butoxide, hydrolyzing in a humidity chamber and drying with nitrogen gas. The TiO2 gel layer formed on the polystyrene dishes was transparent and robust, and its surface was quite flat. Rat hepatocytes inoculated on the TiO2 gel-coated polystyrene dishes gradually accumulated with increasing culture time, and then spontaneously formed many hepatocyte spheroids at 90 ± 21 μm diameter from about 3 days of culture. The diameter of the spheroids increased during the culture, and was 151 ± 43 μm at 14 days of culture. Ammonia removal and albumin secretion by hepatocytes on the TiO2 gel-coated polystyrene dishes were maintained at a high level for at least 14 days of culture compared with on a type I collagen-coated dish and a non-coated polystyrene dish. These results indicate that TiO2 gel is an adequate material for hepatocyte spheroid formation and long-term culture of spheroids.
UR - http://www.scopus.com/inward/record.url?scp=33645885899&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645885899&partnerID=8YFLogxK
U2 - 10.1007/s10856-006-8237-7
DO - 10.1007/s10856-006-8237-7
M3 - Article
C2 - 16617414
AN - SCOPUS:33645885899
VL - 17
SP - 359
EP - 364
JO - Journal of Materials Science: Materials in Electronics
JF - Journal of Materials Science: Materials in Electronics
SN - 0957-4522
IS - 4
ER -