Hepatoma-targeted gene delivery using a tumor cell-specific gene regulation system combined with a human liver cell-specific bionanocapsule

Jeong Hun Kang, Jun Oishi, Jong Hwan Kim, Moeko Ijuin, Riki Toita, Byungdug Jun, Daisuke Asai, Takeshi Mori, Takuro Niidome, Katsuyuki Tanizawa, Shun'ichi Kuroda, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

Hepatoma (hepatocellular carcinoma) is the most common type of malignant tumor originating in the liver and has a relatively low 5-year survival rate. The development of hepatoma-targeted therapy is needed to increase treatment efficiency and to reduce the incidence of undesirable side effects. In this study we developed a novel hepatoma-targeted gene delivery system. The gene delivery system was prepared by combining a human liver cell-specific bionanocapsule (BNC) and a tumor cell-specific gene regulation polymer, which responds to hyperactivated protein kinase C. α in hepatoma cells. The complex of the polymer-DNA with BNCs was delivered into cells and tissues. The developed system showed increased transfection efficiency and resulted in cell-specific gene expression in hepatoma cells and tissues (HuH-7), but no gene expression in normal human hepatocytes or human epidermoid tumor cells (A431). The combination of a tumor cell-specific gene regulation system responding to protein kinase C. α and BNCs showed excellent potential for the selective treatment of hepatomas. The system could be a useful method with applications in hepatoma-specific gene therapy and molecular imaging. From the Clinical Editor: Hepatocellular carcinoma is the most common type of malignant tumor in the liver with a low 5-year survival rate. In this study, a novel hepatoma-targeted gene delivery system was prepared by combining a human liver cell-specific bionanocapsule and a tumor cell-specific gene regulation polymer, which responds to hyperactivated protein kinase C (PKC)a in hepatoma cells. The system could be a useful in hepatoma-specific gene therapy and molecular imaging.

元の言語英語
ページ(範囲)583-589
ページ数7
ジャーナルNanomedicine: Nanotechnology, Biology, and Medicine
6
発行部数4
DOI
出版物ステータス出版済み - 8 2010

Fingerprint

Gene expression
Liver
Tumors
Hepatocellular Carcinoma
Genes
Cells
Protein Kinase C
Molecular imaging
Gene therapy
Neoplasms
Polymers
Gene Transfer Techniques
Proteins
Tissue
Molecular Imaging
Genetic Therapy
DNA
Gene Expression
Transfection
Hepatocytes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Bioengineering
  • Biomedical Engineering
  • Materials Science(all)
  • Medicine (miscellaneous)
  • Pharmaceutical Science

これを引用

Hepatoma-targeted gene delivery using a tumor cell-specific gene regulation system combined with a human liver cell-specific bionanocapsule. / Kang, Jeong Hun; Oishi, Jun; Kim, Jong Hwan; Ijuin, Moeko; Toita, Riki; Jun, Byungdug; Asai, Daisuke; Mori, Takeshi; Niidome, Takuro; Tanizawa, Katsuyuki; Kuroda, Shun'ichi; Katayama, Yoshiki.

:: Nanomedicine: Nanotechnology, Biology, and Medicine, 巻 6, 番号 4, 08.2010, p. 583-589.

研究成果: ジャーナルへの寄稿記事

Kang, Jeong Hun ; Oishi, Jun ; Kim, Jong Hwan ; Ijuin, Moeko ; Toita, Riki ; Jun, Byungdug ; Asai, Daisuke ; Mori, Takeshi ; Niidome, Takuro ; Tanizawa, Katsuyuki ; Kuroda, Shun'ichi ; Katayama, Yoshiki. / Hepatoma-targeted gene delivery using a tumor cell-specific gene regulation system combined with a human liver cell-specific bionanocapsule. :: Nanomedicine: Nanotechnology, Biology, and Medicine. 2010 ; 巻 6, 番号 4. pp. 583-589.
@article{1db9a99dc4bf4ea2b431e9ceace4f9f8,
title = "Hepatoma-targeted gene delivery using a tumor cell-specific gene regulation system combined with a human liver cell-specific bionanocapsule",
abstract = "Hepatoma (hepatocellular carcinoma) is the most common type of malignant tumor originating in the liver and has a relatively low 5-year survival rate. The development of hepatoma-targeted therapy is needed to increase treatment efficiency and to reduce the incidence of undesirable side effects. In this study we developed a novel hepatoma-targeted gene delivery system. The gene delivery system was prepared by combining a human liver cell-specific bionanocapsule (BNC) and a tumor cell-specific gene regulation polymer, which responds to hyperactivated protein kinase C. α in hepatoma cells. The complex of the polymer-DNA with BNCs was delivered into cells and tissues. The developed system showed increased transfection efficiency and resulted in cell-specific gene expression in hepatoma cells and tissues (HuH-7), but no gene expression in normal human hepatocytes or human epidermoid tumor cells (A431). The combination of a tumor cell-specific gene regulation system responding to protein kinase C. α and BNCs showed excellent potential for the selective treatment of hepatomas. The system could be a useful method with applications in hepatoma-specific gene therapy and molecular imaging. From the Clinical Editor: Hepatocellular carcinoma is the most common type of malignant tumor in the liver with a low 5-year survival rate. In this study, a novel hepatoma-targeted gene delivery system was prepared by combining a human liver cell-specific bionanocapsule and a tumor cell-specific gene regulation polymer, which responds to hyperactivated protein kinase C (PKC)a in hepatoma cells. The system could be a useful in hepatoma-specific gene therapy and molecular imaging.",
author = "Kang, {Jeong Hun} and Jun Oishi and Kim, {Jong Hwan} and Moeko Ijuin and Riki Toita and Byungdug Jun and Daisuke Asai and Takeshi Mori and Takuro Niidome and Katsuyuki Tanizawa and Shun'ichi Kuroda and Yoshiki Katayama",
year = "2010",
month = "8",
doi = "10.1016/j.nano.2010.01.007",
language = "English",
volume = "6",
pages = "583--589",
journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",
issn = "1549-9634",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Hepatoma-targeted gene delivery using a tumor cell-specific gene regulation system combined with a human liver cell-specific bionanocapsule

AU - Kang, Jeong Hun

AU - Oishi, Jun

AU - Kim, Jong Hwan

AU - Ijuin, Moeko

AU - Toita, Riki

AU - Jun, Byungdug

AU - Asai, Daisuke

AU - Mori, Takeshi

AU - Niidome, Takuro

AU - Tanizawa, Katsuyuki

AU - Kuroda, Shun'ichi

AU - Katayama, Yoshiki

PY - 2010/8

Y1 - 2010/8

N2 - Hepatoma (hepatocellular carcinoma) is the most common type of malignant tumor originating in the liver and has a relatively low 5-year survival rate. The development of hepatoma-targeted therapy is needed to increase treatment efficiency and to reduce the incidence of undesirable side effects. In this study we developed a novel hepatoma-targeted gene delivery system. The gene delivery system was prepared by combining a human liver cell-specific bionanocapsule (BNC) and a tumor cell-specific gene regulation polymer, which responds to hyperactivated protein kinase C. α in hepatoma cells. The complex of the polymer-DNA with BNCs was delivered into cells and tissues. The developed system showed increased transfection efficiency and resulted in cell-specific gene expression in hepatoma cells and tissues (HuH-7), but no gene expression in normal human hepatocytes or human epidermoid tumor cells (A431). The combination of a tumor cell-specific gene regulation system responding to protein kinase C. α and BNCs showed excellent potential for the selective treatment of hepatomas. The system could be a useful method with applications in hepatoma-specific gene therapy and molecular imaging. From the Clinical Editor: Hepatocellular carcinoma is the most common type of malignant tumor in the liver with a low 5-year survival rate. In this study, a novel hepatoma-targeted gene delivery system was prepared by combining a human liver cell-specific bionanocapsule and a tumor cell-specific gene regulation polymer, which responds to hyperactivated protein kinase C (PKC)a in hepatoma cells. The system could be a useful in hepatoma-specific gene therapy and molecular imaging.

AB - Hepatoma (hepatocellular carcinoma) is the most common type of malignant tumor originating in the liver and has a relatively low 5-year survival rate. The development of hepatoma-targeted therapy is needed to increase treatment efficiency and to reduce the incidence of undesirable side effects. In this study we developed a novel hepatoma-targeted gene delivery system. The gene delivery system was prepared by combining a human liver cell-specific bionanocapsule (BNC) and a tumor cell-specific gene regulation polymer, which responds to hyperactivated protein kinase C. α in hepatoma cells. The complex of the polymer-DNA with BNCs was delivered into cells and tissues. The developed system showed increased transfection efficiency and resulted in cell-specific gene expression in hepatoma cells and tissues (HuH-7), but no gene expression in normal human hepatocytes or human epidermoid tumor cells (A431). The combination of a tumor cell-specific gene regulation system responding to protein kinase C. α and BNCs showed excellent potential for the selective treatment of hepatomas. The system could be a useful method with applications in hepatoma-specific gene therapy and molecular imaging. From the Clinical Editor: Hepatocellular carcinoma is the most common type of malignant tumor in the liver with a low 5-year survival rate. In this study, a novel hepatoma-targeted gene delivery system was prepared by combining a human liver cell-specific bionanocapsule and a tumor cell-specific gene regulation polymer, which responds to hyperactivated protein kinase C (PKC)a in hepatoma cells. The system could be a useful in hepatoma-specific gene therapy and molecular imaging.

UR - http://www.scopus.com/inward/record.url?scp=77955281517&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955281517&partnerID=8YFLogxK

U2 - 10.1016/j.nano.2010.01.007

DO - 10.1016/j.nano.2010.01.007

M3 - Article

C2 - 20138242

AN - SCOPUS:77955281517

VL - 6

SP - 583

EP - 589

JO - Nanomedicine: Nanotechnology, Biology, and Medicine

JF - Nanomedicine: Nanotechnology, Biology, and Medicine

SN - 1549-9634

IS - 4

ER -