Heterologous Production and Glycosylation of Japanese Eel Follitropin Using Silkworm

Sun Mee Hong, Ji Hyun Choi, Sun Jung Jo, Kwan Sik Min, Dae Jung Kim, Jae Man Lee, Takahiro Kusakabe

研究成果: ジャーナルへの寄稿記事

抄録

Follitropin, an important gonadotropin hormone, participates in vitellogenesis and spermatogenesis. Equine chorionic gonadotropin (eCG) can induce gonadotropin hormone activity in non-equid species and exhibits a long biological half-life. Here, we report the production, using silkworm larval and pupal systems, of biologically active recombinant hybrid-type follitropins based on the coding sequence of the eCG C-terminal peptide (CTP) between the mature β- and α-chains of eel. The three constructs, rJeFSH, rJeFSH·eCG, and rJeFSH·2xeCG were produced and verified to be N- or O-glycosylated and secreted mature peptides. Although rJeFSH·eCG contains more elaborate O-linked carbohydrate chains than rJeFSH, it elicited no significant in vitro oocyte maturation, which may be a result of insufficient terminal sialylation of its N-and O-linked carbohydrate chains. Then, a hybrid of rJeFSH·2xeCG extended with two eCG CTP. Furthermore, the receptor binding assay revealed potency of rJeFSH and rJeFSH·2xeCG to be a few folds greater than that of rJeFSH·eCG. The findings of this study will be useful for the development of more efficient GTHs in teleosts, including eels, when various modifications with two or more extended eCG CTP produced by silkworm are included.

元の言語英語
ページ(範囲)745-753
ページ数9
ジャーナルBiotechnology and Bioprocess Engineering
24
発行部数5
DOI
出版物ステータス出版済み - 9 1 2019

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Equine Gonadotropins
Glycosylation
Eels
Bombyx
Follicle Stimulating Hormone
Chorionic Gonadotropin
Peptides
Gonadotropins
Hormones
Carbohydrates
In Vitro Oocyte Maturation Techniques
Vitellogenesis
Spermatogenesis
Half-Life
Assays

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

これを引用

Heterologous Production and Glycosylation of Japanese Eel Follitropin Using Silkworm. / Hong, Sun Mee; Choi, Ji Hyun; Jo, Sun Jung; Min, Kwan Sik; Kim, Dae Jung; Lee, Jae Man; Kusakabe, Takahiro.

:: Biotechnology and Bioprocess Engineering, 巻 24, 番号 5, 01.09.2019, p. 745-753.

研究成果: ジャーナルへの寄稿記事

Hong, Sun Mee ; Choi, Ji Hyun ; Jo, Sun Jung ; Min, Kwan Sik ; Kim, Dae Jung ; Lee, Jae Man ; Kusakabe, Takahiro. / Heterologous Production and Glycosylation of Japanese Eel Follitropin Using Silkworm. :: Biotechnology and Bioprocess Engineering. 2019 ; 巻 24, 番号 5. pp. 745-753.
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