High-level expression of maize phosphoenolpyruvate carboxylase in transgenie rice plants

Maurice S.B. Ku, Sakae Agarie, Mika Nomura, Hiroshi Fukayama, Hiroko Tsuchida, Kazuko Ono, Sakiko Hirose, Seiichi Toki, Mitsue Miyao, Makoto Matsuoka

研究成果: Contribution to journalArticle査読

309 被引用数 (Scopus)

抄録

Using an Agrobacterium-mediated transformation system, we have introduced the intact gene of maize phosphoenolpyruvate carboxylase (PEPC), which catalyzes the initial fixation of atmospheric CO2 in C4 plants into the C3 crop rice. Most transgenic rice plants showed high-level expression of the maize gene; the activities of PEPC in leaves of some transgenic plants were two- to threefold higher than those in maize, and the enzyme accounted for up to 12% of the total leaf soluble protein. RNA gel blot and Southern blot analyses showed that the level of expression of the maize PEPC in transgenic rice plants correlated with the amount of transcript and the copy number of the inserted maize gene. Physiologically, the transgenic plants exhibited reduced O2 inhibition of photosynthesis and photosynthetic rates comparable to those of untransformed plants. The results demonstrate a successful strategy for installing the key biochemical component of the C4 pathway of photosynthesis in C3 plants.

本文言語英語
ページ(範囲)76-81
ページ数6
ジャーナルNature Biotechnology
17
1
DOI
出版ステータス出版済み - 1 1999
外部発表はい

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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