High-level expression of single-chain Fv-Fc fusion protein in serum and egg white of genetically manipulated chickens by using a retroviral vector

Masamichi Kamihira, Ken Ichiro Ono, Kazuhisa Esaka, Ken Ichi Nishijima, Ryoko Kigaku, Hiroyuki Komatsu, Takashi Yamashita, Kenji Kyogoku, Shinji Iijima

研究成果: ジャーナルへの寄稿学術誌査読

105 被引用数 (Scopus)

抄録

We report here the generation of transgenic chickens using a retroviral vector for the production of recombinant proteins. It was found that the transgene expression was suppressed when a Moloney murine leukemia virus-based retroviral vector was injected into chicken embryos at the blastodermal stage. When a concentrated viral solution was injected into the heart of developing embryos after 50 to 60 h of incubation, transgene expression was observed throughout the embryo, including the gonads. For practical production, a retroviral vector encoding an expression cassette of antiprion single-chain Fv fused with the Fc region of human immunoglobulin G1 (scFv-Fc) was injected into chicken embryos. The birds that hatched stably produced scFv-Fc in their serum and eggs at high levels (∼5.6 mg/ml). We obtained transgenic progeny from a transgenic chicken generated with this procedure. The transgene was stably integrated into the chromosomes of transgenic progeny. The transgenic progeny also expressed scFv-Fc in the serum and eggs.

本文言語英語
ページ(範囲)10864-10874
ページ数11
ジャーナルJournal of virology
79
17
DOI
出版ステータス出版済み - 9月 2005

!!!All Science Journal Classification (ASJC) codes

  • 微生物学
  • 免疫学
  • 昆虫科学
  • ウイルス学

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