High-level heterologous expression of fungal cytochrome P450s in Escherichia coli

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

A thorough understanding of the sequence-structure-function relationships of cytochrome P450 (P450) is necessary to better understand the metabolic diversity of living organisms. Significant amounts of pure enzymes are sometimes required for biochemical studies, and their acquisition often relies on the possibility of their heterologous expression. In this study, we performed extensive heterologous expression of fungal P450s in Escherichia coli using 304 P450 isoforms. Using large-scale screening, we confirmed that at least 27 P450s could be expressed with/without simple sequence deletion at the 5' end of cDNAs, which encode the N-terminal hydrophobic domain of the enzyme. Moreover, we identified N-terminal amino acid sequences that can potentially be used to construct chimeric P450s, which could dramatically improve their expression levels even when the expression of the wild-type sequence was unpromising. These findings will help increase the chance of heterologous expression of a variety of fungal and other eukaryotic membrane-bound P450s in E. coli.

元の言語英語
ページ(範囲)289-294
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
438
発行部数2
DOI
出版物ステータス出版済み - 8 23 2013

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Cytochromes
Escherichia coli
Sequence Deletion
Enzymes
Cytochrome P-450 Enzyme System
Amino Acid Sequence
Screening
Protein Isoforms
Complementary DNA
Membranes
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

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