High pressure sensitizes murine erythroleukemia cells to caffeine-induced premature mitosis

M. Matsumoto, T. Yamaguchi, K. Nakazono, Y. Fukumaki, S. Terada

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Murine erythroleukemia (MEL) cells were exposed to a high pressure of 80 MPa or aphidicolin (APH), DNA polymerase inhibitor. The effects of caffeine on cell cycle were examined using these cells. During the culture of 80 MPa-treated MEL cells at atmospheric pressure, the cells arrested in the G2 phase, and cyclin B and hyperphosphorylated p34(cdc2) were accumulated. Namely, maturation promoting factor (MPF) composed of p34(cdc2) and cyclin B was inactive. However, upon exposure to caffeine, these cells entered prematurely into mitosis by activating MPF. Caffeine-induced premature mitosis was suppressed by butyrolactone I and orthovanadate. On the other hand, APH-treated MEL cells, which were not exposed to 80 MPa, were not so sensitive to caffeine-induced premature mitosis despite cyclin B accumulation. In this case, dephosphorylation of p34(cdc2) was not induced by caffeine. Interestingly, caffeine-induced premature mitosis in the 80 MPa-treated cells was also suppressed by APH. These results suggest that the premature mitosis of 80 MPa-treated MEL cells by caffeine is induced by active MPF, and that APH-sensitive molecules such as DNA polymerase may also play an important role in the checkpoint that controls the transition from G2 to M phase.

元の言語英語
ページ(範囲)329-336
ページ数8
ジャーナルJapanese Journal of Physiology
50
発行部数3
DOI
出版物ステータス出版済み - 1 1 2000

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Leukemia, Erythroblastic, Acute
Caffeine
Mitosis
Aphidicolin
Pressure
Maturation-Promoting Factor
Cyclin B
Nucleic Acid Synthesis Inhibitors
Atmospheric Pressure
Vanadates
G2 Phase
DNA-Directed DNA Polymerase
Cell Division
Cell Cycle

All Science Journal Classification (ASJC) codes

  • Physiology

これを引用

High pressure sensitizes murine erythroleukemia cells to caffeine-induced premature mitosis. / Matsumoto, M.; Yamaguchi, T.; Nakazono, K.; Fukumaki, Y.; Terada, S.

:: Japanese Journal of Physiology, 巻 50, 番号 3, 01.01.2000, p. 329-336.

研究成果: ジャーナルへの寄稿記事

Matsumoto, M. ; Yamaguchi, T. ; Nakazono, K. ; Fukumaki, Y. ; Terada, S. / High pressure sensitizes murine erythroleukemia cells to caffeine-induced premature mitosis. :: Japanese Journal of Physiology. 2000 ; 巻 50, 番号 3. pp. 329-336.
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AU - Terada, S.

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