High-resolution analysis of polyprenols by supercritical fluid chromatography

T. Bamba, E. Fukusaki, S. Kajiyama, K. Ute, T. Kitayama, A. Kobayashi

研究成果: ジャーナルへの寄稿記事

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A high-resolution analysis of polyprenol mixtures was achieved by supercritical fluid chromatography (SFC). The separation of polyprenols was examined on an octadecylsilane-packed column with liquid carbon dioxide as the mobile phase and ethanol as modifier. Using this chromatography system, the resolution of separation (Rs) between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) was two times higher than that using conventional reversed-phase high-performance liquid chromatography. Our SFC technique allows the advantage of baseline separation of polyprenol samples containing hydrophobic components such as terpenes or fatty acids that are unfavorable for good separation. This method is very useful for the analysis of structurally close polyprenol analogues of rubber plant metabolites.

元の言語英語
ページ(範囲)113-117
ページ数5
ジャーナルJournal of Chromatography A
911
発行部数1
DOI
出版物ステータス出版済み - 3 9 2001
外部発表Yes

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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