High speed DNA denaturation using microheating devices

Masayuki Furuhashi, Yukihiro Okamoto, Daisuke Onoshima, Takahito Ohshiro, Sou Ryuzaki, Kazumichi Yokota, Makusu Tsutsui, Masateru Taniguchi, Kazuhiko Nakatani, Yoshinobu Baba, Tomoji Kawai

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

Denaturation is a first step for further treatment of DNA and is expected to be carried out rapidly on an integrated chip. A microheater is a promising device for the denaturation because of easiness for fabrication and operation. In the present study, we fabricated a microheater and thermometers on a coverslip and investigated response of temperature to application of voltage. In addition, our experiment and simulation proved local heating at an aimed area. Finally, we demonstrated denaturation of DNA in buffer solution, the result of which proved that the DNA around the heater denatured within 60 ms.

本文言語英語
論文番号023112
ジャーナルApplied Physics Letters
103
2
DOI
出版ステータス出版済み - 7 8 2013
外部発表はい

All Science Journal Classification (ASJC) codes

  • 物理学および天文学(その他)

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