High-throughput detection of protein kinase activities in cell lysate based on the aggregation of gold nanoparticles with peptides

Yoshiki Katayama, Hirotaro Kitazaki, Jeong Hun Kang, Xiaoming Han, Takeshi Mori, Takuro Niidome

    研究成果: 書籍/レポート タイプへの寄稿会議への寄与

    抄録

    Cationic peptides were used as coagulant of gold nano-particle (GNP, 20 nm) for the monitoring cellular protein kinase activities. If cationic peptides, which are designed as specific substrates to target protein kinases, were added into the GNP dispersion, the color of the dispersion changed from red to blue due to the aggregation. On the other hand, if the peptide was phosphorylated with target protein kinase, addition of the phosphorylated peptide didn't change the color of the GNP dispersion. Thus, this system can be a rapid assay of protein kinase activity. The method was applicable to the assay of intracellular protein kinase by using cell lysate. In addition, we applied the system for high-throughput screening of protein kinase inhibitor using 1300 kinds of chemical library. The result indicated that the method was also useful for the drug discovery.

    本文言語英語
    ホスト出版物のタイトルBiological Imaging and Sensing Using Nanoparticle Assemblies
    ページ39-44
    ページ数6
    出版ステータス出版済み - 10月 15 2010
    イベント2009 MRS Fall Meeting - Boston, MA, 米国
    継続期間: 11月 30 200912月 4 2009

    出版物シリーズ

    名前Materials Research Society Symposium Proceedings
    1241
    ISSN(印刷版)0272-9172

    その他

    その他2009 MRS Fall Meeting
    国/地域米国
    CityBoston, MA
    Period11/30/0912/4/09

    !!!All Science Journal Classification (ASJC) codes

    • 材料科学(全般)
    • 凝縮系物理学
    • 材料力学
    • 機械工学

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