High-throughput label-free molecular fingerprinting flow cytometry

Kotaro Hiramatsu, Takuro Ideguchi, Yusuke Yonamine, Sang Wook Lee, Yizhi Luo, Kazuki Hashimoto, Takuro Ito, Misa Hase, Jee Woong Park, Yusuke Kasai, Shinya Sakuma, Takeshi Hayakawa, Fumihito Arai, Yu Hoshino, Keisuke Goda

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

Flow cytometry is an indispensable tool in biology for counting and analyzing single cells in large heterogeneous populations. However, it predominantly relies on fluorescent labeling to differentiate cells and, hence, comes with several fundamental drawbacks. Here, we present a high-throughput Raman flow cytometer on a microfluidic chip that chemically probes single live cells in a label-free manner. It is based on a rapid-scan Fourier-transform coherent anti-Stokes Raman scattering spectrometer as an optical interrogator, enabling us to obtain the broadband molecular vibrational spectrum of every single cell in the fingerprint region (400 to 1600 cm −1 ) with a record-high throughput of ~2000 events/s. As a practical application of the method not feasible with conventional flow cytometry, we demonstrate high-throughput label-free single-cell analysis of the astaxanthin productivity and photosynthetic dynamics of Haematococcus lacustris.

元の言語英語
記事番号eaau0241
ジャーナルScience Advances
5
発行部数1
DOI
出版物ステータス出版済み - 1 16 2019

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Flow cytometry
Labels
Throughput
Vibrational spectra
Microfluidics
Labeling
Spectrometers
Raman scattering
Fourier transforms
Productivity

All Science Journal Classification (ASJC) codes

  • General

これを引用

Hiramatsu, K., Ideguchi, T., Yonamine, Y., Lee, S. W., Luo, Y., Hashimoto, K., ... Goda, K. (2019). High-throughput label-free molecular fingerprinting flow cytometry. Science Advances, 5(1), [eaau0241]. https://doi.org/10.1126/sciadv.aau0241

High-throughput label-free molecular fingerprinting flow cytometry. / Hiramatsu, Kotaro; Ideguchi, Takuro; Yonamine, Yusuke; Lee, Sang Wook; Luo, Yizhi; Hashimoto, Kazuki; Ito, Takuro; Hase, Misa; Park, Jee Woong; Kasai, Yusuke; Sakuma, Shinya; Hayakawa, Takeshi; Arai, Fumihito; Hoshino, Yu; Goda, Keisuke.

:: Science Advances, 巻 5, 番号 1, eaau0241, 16.01.2019.

研究成果: ジャーナルへの寄稿記事

Hiramatsu, K, Ideguchi, T, Yonamine, Y, Lee, SW, Luo, Y, Hashimoto, K, Ito, T, Hase, M, Park, JW, Kasai, Y, Sakuma, S, Hayakawa, T, Arai, F, Hoshino, Y & Goda, K 2019, 'High-throughput label-free molecular fingerprinting flow cytometry', Science Advances, 巻. 5, 番号 1, eaau0241. https://doi.org/10.1126/sciadv.aau0241
Hiramatsu K, Ideguchi T, Yonamine Y, Lee SW, Luo Y, Hashimoto K その他. High-throughput label-free molecular fingerprinting flow cytometry. Science Advances. 2019 1 16;5(1). eaau0241. https://doi.org/10.1126/sciadv.aau0241
Hiramatsu, Kotaro ; Ideguchi, Takuro ; Yonamine, Yusuke ; Lee, Sang Wook ; Luo, Yizhi ; Hashimoto, Kazuki ; Ito, Takuro ; Hase, Misa ; Park, Jee Woong ; Kasai, Yusuke ; Sakuma, Shinya ; Hayakawa, Takeshi ; Arai, Fumihito ; Hoshino, Yu ; Goda, Keisuke. / High-throughput label-free molecular fingerprinting flow cytometry. :: Science Advances. 2019 ; 巻 5, 番号 1.
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