High throughput purification device for gene delivery multifunctional envelope-type nanodevice

Daisuke Shigenaka, Masami Ukawa, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Hidetaka Akita, Hideyoshi Harashima, Yoshinobu Baba

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

1 被引用数 (Scopus)

抄録

Multifunctional envelope-type nanodevices (MEND) developed by Harashima et al. is one of the novel non-viral DNA vectors expected as a safe gene delivery system. Our group has already attained fast and easy preparation of MEND by using the microfluidic device. However, purification of MEND should be performed in a high-throughput manner because prepared MEND contains impurities such as plasmid-cationic polymer complex. In this paper, we report a novel purification method for MEND by free flow electrophoresis based on microfluidic device, which purification principle is based on the zeta potential difference between MEND and impurities.

本文言語英語
ホスト出版物のタイトルProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
出版社Chemical and Biological Microsystems Society
ページ1546-1548
ページ数3
ISBN(印刷版)9780979806452
出版ステータス出版済み - 1 1 2012
外部発表はい
イベント16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, 日本
継続期間: 10 28 201211 1 2012

出版物シリーズ

名前Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

その他

その他16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country日本
CityOkinawa
Period10/28/1211/1/12

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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