High-throughput study of protein-surface interactions using a surface plasmon resonance imaging apparatus

Yusuke Arima, Rika Ishii, Isao Hirata, Hiroo Iwata

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

1 被引用数 (Scopus)

抄録

Protein adsorption at the liquid-solid interface is critically important for the development of biomedical materials. Surface plasmon resonance (SPR) has been widely used in real-time monitoring of protein adsorption behavior on surfaces. However, the number of combinations of protein-surface interactions that can be measured in a single experiment is limited by a conventional SPR apparatus. We assembled an SPR imaging apparatus for high-throughput studies of many sets of protein-surface interactions. In addition, SPR sensor surfaces with arrayed spots carrying different surface properties were prepared by taking advantage of self-assembled monolayers of alkanethiols. The combination of the SPR imaging apparatus and the arrayed sensor surfaces allows for multiple and real-time monitoring of protein-surface interactions.

本文言語英語
ホスト出版物のタイトルProteins at Interfaces III State of the Art
出版社American Chemical Society
ページ695-708
ページ数14
ISBN(印刷版)9780841227965
DOI
出版ステータス出版済み - 12 12 2012
外部発表はい

出版物シリーズ

名前ACS Symposium Series
1120
ISSN(印刷版)0097-6156
ISSN(電子版)1947-5918

All Science Journal Classification (ASJC) codes

  • 化学 (全般)
  • 化学工学(全般)

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