Higher chromatin mobility supports totipotency and precedes pluripotency in vivo

Ana Bošković, André Eid, Julien Pontabry, Takashi Ishiuchi, Coralie Spiegelhalter, Edupuganti V.S. Raghu Ram, Eran Meshorer, Maria Elena Torres-Padilla

研究成果: ジャーナルへの寄稿学術誌査読

106 被引用数 (Scopus)


The fusion of the gametes upon fertilization results in the formation of a totipotent cell. Embryonic chromatin is expected to be able to support a large degree of plasticity. However, whether this plasticity relies on a particular conformation of the embryonic chromatin is unknown. Moreover, whether chromatin plasticity is functionally linked to cellular potency has not been addressed. Here, we adapted fluorescence recovery after photobleaching (FRAP) in the developing mouse embryo and show that mobility of the core histones H2A, H3.1, and H3.2 is unusually high in two-cell stage embryos and decreases as development proceeds. The transition toward pluripotency is accompanied by a decrease in histone mobility, and, upon lineage allocation, pluripotent cells retain higher mobility than the differentiated trophectoderm. Importantly, totipotent two-cell-like embryonic stem cells also display high core histone mobility, implying that reprogramming toward totipotency entails changes in chromatin mobility. Our data suggest that changes in chromatin dynamics underlie the transitions in cellular plasticity and that higher chromatin mobility is at the nuclear foundations of totipotency.

ジャーナルGenes and Development
出版ステータス出版済み - 2014

!!!All Science Journal Classification (ASJC) codes

  • 遺伝学
  • 発生生物学


「Higher chromatin mobility supports totipotency and precedes pluripotency in vivo」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。