Highly Selective Rational Design of Peptide-Based Surface Plasmon Resonance Sensor for Direct Determination of 2,4,6-trinitrotoluene (TNT) Explosive

Jin Wang, Masaki Muto, Rui Yatabe, Yusuke Tahara, Takeshi Onodera, Masayoshi Tanaka, Mina Okochi, Kiyoshi Toko

研究成果: Contribution to journalArticle査読

27 被引用数 (Scopus)

抄録

In this study, 2,4,6-trinitrotoluene (TNT) binding peptide was synthesized and screened for TNT specific detection using surface plasmon resonance (SPR) sensor. The TNT binding peptide was rational design and synthesized through amino acid sequence from complementarity determining region (CDR) in the anti-TNT monoclonal antibody, which was produced from hybridoma cell using TNP-KLH conjugate as antigen. Three TNT binding peptide sequences were obtained from the heavy chain of CDR1 named TNTHCDR1, TNTHCDR2 from CDR2 and TNTHCDR3 from CDR3 of anti-TNT antibody. Screening process of three candidate peptides were carried out by using the SPR sensor with direct determination, which the peptide was directly immobilized on the sensor chip CM7 surface through amine coupling reaction. The results demonstrated that peptide TNTHCDR3 was determined as TNT binding peptide and no non-specific binding was observed. The selectivity of TNT binding peptide TNTHCDR3 was also testified by six kinds of TNT analogues. The results indicated the specific binding between TNT and peptide TNTHCDR3.

本文言語英語
ページ(範囲)279-284
ページ数6
ジャーナルSensors and Actuators, B: Chemical
264
DOI
出版ステータス出版済み - 7 1 2018

All Science Journal Classification (ASJC) codes

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

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