Highly sensitive and quantitative evaluation of the EGFR T790M mutation by nanofluidic digital PCR

Eiji Iwama, Koichi Takayama, Taishi Harada, Isamu Okamoto, Fumihiko Ookubo, Junji Kishimoto, Eishi Baba, Yoshinao Oda, Yoichi Nakanishi

研究成果: ジャーナルへの寄稿記事

18 引用 (Scopus)

抄録

The mutation of T790M in EGFR is a major mechanism of resistance to treatment with EGFR-TKIs. Only qualitative detection (presence or absence) of T790M has been described to date, however. Digital PCR (dPCR) analysis has recently been applied to the quantitative detection of target molecules in cancer with high sensitivity. In the present study, 25 tumor samples (13 obtained before and 12 after EGFR-TKI treatment) from 18 NSCLC patients with activating EGFR mutations were evaluated for T790M with dPCR. The ratio of the number of T790M alleles to that of activating mutation alleles (T/A) was determined. dPCR detected T790M in all 25 samples. Although T790M was present in all pre-TKI samples from 13 patients, 10 of these patients had a low T/A ratio and manifested substantial tumor shrinkage during treatment with EGFR-TKIs. In six of seven patients for whom both pre- and post-TKI samples were available, the T/A ratio increased markedly during EGFR-TKI treatment. Highly sensitive dPCR thus detected T790M in all NSCLC patients harboring activating EGFR mutations whether or not they had received EGFR-TKI treatment. Not only highly sensitive but also quantitative detection of T790M is important for evaluation of the contribution of T790M to EGFR-TKI resistance.

元の言語英語
ページ(範囲)20466-20473
ページ数8
ジャーナルOncotarget
6
発行部数24
DOI
出版物ステータス出版済み - 1 1 2015

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Polymerase Chain Reaction
Mutation
Alleles
Therapeutics
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology

これを引用

Highly sensitive and quantitative evaluation of the EGFR T790M mutation by nanofluidic digital PCR. / Iwama, Eiji; Takayama, Koichi; Harada, Taishi; Okamoto, Isamu; Ookubo, Fumihiko; Kishimoto, Junji; Baba, Eishi; Oda, Yoshinao; Nakanishi, Yoichi.

:: Oncotarget, 巻 6, 番号 24, 01.01.2015, p. 20466-20473.

研究成果: ジャーナルへの寄稿記事

Iwama, Eiji ; Takayama, Koichi ; Harada, Taishi ; Okamoto, Isamu ; Ookubo, Fumihiko ; Kishimoto, Junji ; Baba, Eishi ; Oda, Yoshinao ; Nakanishi, Yoichi. / Highly sensitive and quantitative evaluation of the EGFR T790M mutation by nanofluidic digital PCR. :: Oncotarget. 2015 ; 巻 6, 番号 24. pp. 20466-20473.
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