Highly sensitive detection of 2,4,6-trinitrotoluene(TNT) using poly(vinylamine-co-N-vinylformamide)based surface plasmon resonance (SPR) immunosensor

研究成果: Contribution to journalArticle査読

10 被引用数 (Scopus)

抄録

In this paper, we describe the fabrication of a novel poly-(vinylamine-co- Nvinylformamide) (poly-(VAm-co-NVF))-based surface plasmon resonance (SPR) sensor chip supported by a self-assembled monolayer (SAM) of alkanethiol and its use in the highly sensitive detection of 2,4,6-trinitrotoluene (TNT), which is a typical explosive. 2,4-dinitrophenyl-glycine (DNP-GIy) was immobilized to amino groups on the side chain of poly-vinylamine (poly-VAm) on the sensor surface. The fabricated surface showed a high response for flow of anti-TNT antibodies. However, it was found that the response included the nonspecific adsorption of anti-TNT antibodies, because unreacted amino groups of the poly-VAm were positively charged and it caused nonspecific adsorption due to electrostatic interaction. Then, the number of amino groups was reduced by making low-hydrolyzed poly-N-vinylformamideipoly-NVF), i.e., poly(VAm-co-NVF) -poly-NVF is a precursor of poly-VAm, and nonspecific adsorption was controlled. The limit of detection (LOD) of TNT was 28 ppt using the sensor chip fabricated with 23% hydrolyzed poly-(VAm-co-NVF) by inhibition assay.

本文言語英語
ページ(範囲)45-56
ページ数12
ジャーナルSensors and Materials
25
1
出版ステータス出版済み - 2013

All Science Journal Classification (ASJC) codes

  • 器械工学
  • 材料科学(全般)

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