Highly Sensitive Simultaneous Determination of Prostaglandins by Using HPLC/Laser Induced Fluorescence (LIF).

Shinya Sakae, Akira Harata, Takehiko Kitamori, Tsuguo Sawada, Akira Okubo, Shozo Toda, Takao Shimizu, Hideo Watanabe

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

We have developed a laser induced fluorometric (LIF) detection method combined with HPLC and an analytical procedure for prostaglandins (PGs) which allows analysis of practical samples without interference from admixtures and residual reagents. We were able to derivatize PGs with 9-anthryldiazomethtane (ADAM) at 4°C in the dark after 12 hours. This method was applied to analysis of human urine (20 μL). Clear separation and detection of PGs at fmole levels could be achieved. The lower detection limit was 1.0 fmole for PGF1α.

本文言語英語
ページ(範囲)957-958
ページ数2
ジャーナルanalytical sciences
7
DOI
出版ステータス出版済み - 1 1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分析化学

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