Histological analysis of neurodegeneration in the mouse brain

Hiroo Yamaguchi, Jie Shen

研究成果: Chapter in Book/Report/Conference proceedingChapter

抄録

Neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD) are characterized by chronic and progressive neuronal loss. Being able to detect and quantify neurodegeneration is the first step to identify mechanisms underlying neuronal cell death and to develop novel therapeutic strategies. In this chapter, we describe a practical method for detecting and quantifying neurodegeneration in adult and aging mouse brains based on protocols developed in our laboratory over the last decade. We include protocols on sample preparation, immunohistochemical analysis, and stereological methods for counting neurons using examples of AD and PD mouse models. We also describe how to use Fluoro-Jade staining and terminal deoxynucleotidyl transferase dUTP nick end labeling to detect degenerating neurons and apoptotic cells, respectively, and how to use specific proteins as early markers of neurodegeneration.

本文言語英語
ホスト出版物のタイトルNecrosis
ホスト出版物のサブタイトルMethods and Protocols
ページ91-113
ページ数23
1004
DOI
出版ステータス出版済み - 9 5 2013
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
1004
ISSN(印刷版)1064-3745

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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