Histone H3.3 sub-variant H3mm7 is required for normal skeletal muscle regeneration

Akihito Harada, Kazumitsu Maehara, Yusuke Ono, Hiroyuki Taguchi, Kiyoshi Yoshioka, Yasuo Kitajima, Yan Xie, Yuko Sato, Takeshi Iwasaki, Jumpei Nogami, Seiji Okada, Tetsuro Komatsu, Yuichiro Semba, Tatsuya Takemoto, Hiroshi Kimura, Hitoshi Kurumizaka, Yasuyuki Ohkawa

研究成果: ジャーナルへの寄稿記事

抄録

Regulation of gene expression requires selective incorporation of histone H3 variant H3.3 into chromatin. Histone H3.3 has several subsidiary variants but their functions are unclear. Here we characterize the function of histone H3.3 sub-variant, H3mm7, which is expressed in skeletal muscle satellite cells. H3mm7 knockout mice demonstrate an essential role of H3mm7 in skeletal muscle regeneration. Chromatin analysis reveals that H3mm7 facilitates transcription by forming an open chromatin structure around promoter regions including those of myogenic genes. The crystal structure of the nucleosome containing H3mm7 reveals that, unlike the S57 residue of other H3 proteins, the H3mm7-specific A57 residue cannot form a hydrogen bond with the R40 residue of the cognate H4 molecule. Consequently, the H3mm7 nucleosome is unstable in vitro and exhibited higher mobility in vivo compared with the H3.3 nucleosome. We conclude that the unstable H3mm7 nucleosome may be required for proper skeletal muscle differentiation.

元の言語英語
記事番号1400
ジャーナルNature communications
9
発行部数1
DOI
出版物ステータス出版済み - 12 1 2018

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skeletal muscle
chromatin
Nucleosomes
regeneration
Histones
Muscle
Regeneration
Skeletal Muscle
Chromatin
knockout mice
Allura Red AC Dye
subsidiaries
Skeletal Muscle Satellite Cells
gene expression
genes
Gene Expression Regulation
Transcription
Genetic Promoter Regions
Gene expression
Knockout Mice

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

これを引用

Histone H3.3 sub-variant H3mm7 is required for normal skeletal muscle regeneration. / Harada, Akihito; Maehara, Kazumitsu; Ono, Yusuke; Taguchi, Hiroyuki; Yoshioka, Kiyoshi; Kitajima, Yasuo; Xie, Yan; Sato, Yuko; Iwasaki, Takeshi; Nogami, Jumpei; Okada, Seiji; Komatsu, Tetsuro; Semba, Yuichiro; Takemoto, Tatsuya; Kimura, Hiroshi; Kurumizaka, Hitoshi; Ohkawa, Yasuyuki.

:: Nature communications, 巻 9, 番号 1, 1400, 01.12.2018.

研究成果: ジャーナルへの寄稿記事

Harada, A, Maehara, K, Ono, Y, Taguchi, H, Yoshioka, K, Kitajima, Y, Xie, Y, Sato, Y, Iwasaki, T, Nogami, J, Okada, S, Komatsu, T, Semba, Y, Takemoto, T, Kimura, H, Kurumizaka, H & Ohkawa, Y 2018, 'Histone H3.3 sub-variant H3mm7 is required for normal skeletal muscle regeneration', Nature communications, 巻. 9, 番号 1, 1400. https://doi.org/10.1038/s41467-018-03845-1
Harada, Akihito ; Maehara, Kazumitsu ; Ono, Yusuke ; Taguchi, Hiroyuki ; Yoshioka, Kiyoshi ; Kitajima, Yasuo ; Xie, Yan ; Sato, Yuko ; Iwasaki, Takeshi ; Nogami, Jumpei ; Okada, Seiji ; Komatsu, Tetsuro ; Semba, Yuichiro ; Takemoto, Tatsuya ; Kimura, Hiroshi ; Kurumizaka, Hitoshi ; Ohkawa, Yasuyuki. / Histone H3.3 sub-variant H3mm7 is required for normal skeletal muscle regeneration. :: Nature communications. 2018 ; 巻 9, 番号 1.
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