Hopping and stalling of processive molecular motors

Yasuhiro Imafuku, Neil Thomas, Katsuhisa Tawada

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

When a two-headed molecular motor such as kinesin is attached to its track by just a single head in the presence of an applied load, thermally activated head detachment followed by rapid re-attachment at another binding site can cause the motor to 'hop' backwards. Such hopping, on its own, would produce a linear force-velocity relation. However, for kinesin, we must incorporate hopping into the motor's alternating-head scheme, where we expect it to be most important for the state prior to neck-linker docking. We show that hopping can account for the backward steps, run length and stalling of conventional kinesin. In particular, although hopping does not hydrolyse ATP, we find that the hopping rate obeys the same Michaelis-Menten relation as the ATP hydrolysis rate. Hopping can also account for the reduced processivity observed in kinesins with mutations in their tubulin-binding loop. Indeed, it may provide a general mechanism for the breakdown of perfect processivity in two-headed molecular motors.

元の言語英語
ページ(範囲)43-49
ページ数7
ジャーナルJournal of Theoretical Biology
261
発行部数1
DOI
出版物ステータス出版済み - 11 7 2009

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Molecular Motor
Kinesin
kinesin
Run Length
Docking
Adenosinetriphosphate
Head
Breakdown
Mutation
Adenosine Triphosphate
Humulus
hops
Binding sites
Tubulin
tubulin
neck
binding sites
Hydrolysis
Neck
hydrolysis

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Modelling and Simulation
  • Statistics and Probability
  • Applied Mathematics

これを引用

Hopping and stalling of processive molecular motors. / Imafuku, Yasuhiro; Thomas, Neil; Tawada, Katsuhisa.

:: Journal of Theoretical Biology, 巻 261, 番号 1, 07.11.2009, p. 43-49.

研究成果: ジャーナルへの寄稿記事

Imafuku, Yasuhiro ; Thomas, Neil ; Tawada, Katsuhisa. / Hopping and stalling of processive molecular motors. :: Journal of Theoretical Biology. 2009 ; 巻 261, 番号 1. pp. 43-49.
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