Horseshoe crab hemocyte-derived antimicrobial polypeptides, tachystatins, with sequence similarity to spider neurotoxins

Tsukasa Osaki, Miyuki Omotezako, Ranko Nagayama, Michimasa Hirata, Sadaaki Iwanaga, Jiro Kasahara, Junji Hattori, Isao Ito, Hiroyuki Sugiyama, Shun Ichiro Kawabata

研究成果: ジャーナルへの寄稿記事

78 引用 (Scopus)

抄録

Antimicrobial peptides, named tachystatins A, B, and C, were identified from hemocytes of the horseshoe crab Tachypleus tridentatus. Tachystatins exhibited a broad spectrum of antimicrobial activity against Gram-negative and Gram-positive bacteria and fungi. Of these tachystatins, tachystatin C was most effective. Tachystatin A is homologous to tachystatin B, but tachystatin C has no significant sequence similarity to tachystatins A and B. Tachystatins A and B showed sequence similarity to ω-agatoxin-IVA of funnel web spider venom, a potent blocker of voltage-dependent calcium channels. However, they exhibited no blocking activity of the P-type calcium channel in rat Purkinje cells. Tachystatin C also showed sequence similarity to several insecticidal neurotoxins of spider venoms. Tachystatins A, B, and C bound significantly to chitin. A causal relationship was observed between chitin binding activity and antifungal activity. Tachystatins caused morphological changes against a budding yeast, and tachystatin C had a strong cell lysis activity. The septum between mother cell and bud, a chitin-rich region, was stained by fluorescence-labeled tachystatin C, suggesting that the primary recognizing substance on the cell wall is chitin. As horseshoe crab is a close relative of the spider, tachystatins and spider neurotoxins may have evolved from a common ancestral peptide, with adaptive functions.

元の言語英語
ページ(範囲)26172-26178
ページ数7
ジャーナルJournal of Biological Chemistry
274
発行部数37
DOI
出版物ステータス出版済み - 9 10 1999

Fingerprint

Horseshoe Crabs
Hemocytes
Spiders
Neurotoxins
Chitin
Peptides
Agatoxins
P-Type Calcium Channels
Spider Venoms
Saccharomycetales
Purkinje Cells
Gram-Positive Bacteria
Calcium Channels
Fungi
Yeast
Cell Wall
Rats
Bacteria
Stem Cells
Fluorescence

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Horseshoe crab hemocyte-derived antimicrobial polypeptides, tachystatins, with sequence similarity to spider neurotoxins. / Osaki, Tsukasa; Omotezako, Miyuki; Nagayama, Ranko; Hirata, Michimasa; Iwanaga, Sadaaki; Kasahara, Jiro; Hattori, Junji; Ito, Isao; Sugiyama, Hiroyuki; Kawabata, Shun Ichiro.

:: Journal of Biological Chemistry, 巻 274, 番号 37, 10.09.1999, p. 26172-26178.

研究成果: ジャーナルへの寄稿記事

Osaki, T, Omotezako, M, Nagayama, R, Hirata, M, Iwanaga, S, Kasahara, J, Hattori, J, Ito, I, Sugiyama, H & Kawabata, SI 1999, 'Horseshoe crab hemocyte-derived antimicrobial polypeptides, tachystatins, with sequence similarity to spider neurotoxins', Journal of Biological Chemistry, 巻. 274, 番号 37, pp. 26172-26178. https://doi.org/10.1074/jbc.274.37.26172
Osaki, Tsukasa ; Omotezako, Miyuki ; Nagayama, Ranko ; Hirata, Michimasa ; Iwanaga, Sadaaki ; Kasahara, Jiro ; Hattori, Junji ; Ito, Isao ; Sugiyama, Hiroyuki ; Kawabata, Shun Ichiro. / Horseshoe crab hemocyte-derived antimicrobial polypeptides, tachystatins, with sequence similarity to spider neurotoxins. :: Journal of Biological Chemistry. 1999 ; 巻 274, 番号 37. pp. 26172-26178.
@article{d64f61546eb4452096bd65364337eeea,
title = "Horseshoe crab hemocyte-derived antimicrobial polypeptides, tachystatins, with sequence similarity to spider neurotoxins",
abstract = "Antimicrobial peptides, named tachystatins A, B, and C, were identified from hemocytes of the horseshoe crab Tachypleus tridentatus. Tachystatins exhibited a broad spectrum of antimicrobial activity against Gram-negative and Gram-positive bacteria and fungi. Of these tachystatins, tachystatin C was most effective. Tachystatin A is homologous to tachystatin B, but tachystatin C has no significant sequence similarity to tachystatins A and B. Tachystatins A and B showed sequence similarity to ω-agatoxin-IVA of funnel web spider venom, a potent blocker of voltage-dependent calcium channels. However, they exhibited no blocking activity of the P-type calcium channel in rat Purkinje cells. Tachystatin C also showed sequence similarity to several insecticidal neurotoxins of spider venoms. Tachystatins A, B, and C bound significantly to chitin. A causal relationship was observed between chitin binding activity and antifungal activity. Tachystatins caused morphological changes against a budding yeast, and tachystatin C had a strong cell lysis activity. The septum between mother cell and bud, a chitin-rich region, was stained by fluorescence-labeled tachystatin C, suggesting that the primary recognizing substance on the cell wall is chitin. As horseshoe crab is a close relative of the spider, tachystatins and spider neurotoxins may have evolved from a common ancestral peptide, with adaptive functions.",
author = "Tsukasa Osaki and Miyuki Omotezako and Ranko Nagayama and Michimasa Hirata and Sadaaki Iwanaga and Jiro Kasahara and Junji Hattori and Isao Ito and Hiroyuki Sugiyama and Kawabata, {Shun Ichiro}",
year = "1999",
month = "9",
day = "10",
doi = "10.1074/jbc.274.37.26172",
language = "English",
volume = "274",
pages = "26172--26178",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "37",

}

TY - JOUR

T1 - Horseshoe crab hemocyte-derived antimicrobial polypeptides, tachystatins, with sequence similarity to spider neurotoxins

AU - Osaki, Tsukasa

AU - Omotezako, Miyuki

AU - Nagayama, Ranko

AU - Hirata, Michimasa

AU - Iwanaga, Sadaaki

AU - Kasahara, Jiro

AU - Hattori, Junji

AU - Ito, Isao

AU - Sugiyama, Hiroyuki

AU - Kawabata, Shun Ichiro

PY - 1999/9/10

Y1 - 1999/9/10

N2 - Antimicrobial peptides, named tachystatins A, B, and C, were identified from hemocytes of the horseshoe crab Tachypleus tridentatus. Tachystatins exhibited a broad spectrum of antimicrobial activity against Gram-negative and Gram-positive bacteria and fungi. Of these tachystatins, tachystatin C was most effective. Tachystatin A is homologous to tachystatin B, but tachystatin C has no significant sequence similarity to tachystatins A and B. Tachystatins A and B showed sequence similarity to ω-agatoxin-IVA of funnel web spider venom, a potent blocker of voltage-dependent calcium channels. However, they exhibited no blocking activity of the P-type calcium channel in rat Purkinje cells. Tachystatin C also showed sequence similarity to several insecticidal neurotoxins of spider venoms. Tachystatins A, B, and C bound significantly to chitin. A causal relationship was observed between chitin binding activity and antifungal activity. Tachystatins caused morphological changes against a budding yeast, and tachystatin C had a strong cell lysis activity. The septum between mother cell and bud, a chitin-rich region, was stained by fluorescence-labeled tachystatin C, suggesting that the primary recognizing substance on the cell wall is chitin. As horseshoe crab is a close relative of the spider, tachystatins and spider neurotoxins may have evolved from a common ancestral peptide, with adaptive functions.

AB - Antimicrobial peptides, named tachystatins A, B, and C, were identified from hemocytes of the horseshoe crab Tachypleus tridentatus. Tachystatins exhibited a broad spectrum of antimicrobial activity against Gram-negative and Gram-positive bacteria and fungi. Of these tachystatins, tachystatin C was most effective. Tachystatin A is homologous to tachystatin B, but tachystatin C has no significant sequence similarity to tachystatins A and B. Tachystatins A and B showed sequence similarity to ω-agatoxin-IVA of funnel web spider venom, a potent blocker of voltage-dependent calcium channels. However, they exhibited no blocking activity of the P-type calcium channel in rat Purkinje cells. Tachystatin C also showed sequence similarity to several insecticidal neurotoxins of spider venoms. Tachystatins A, B, and C bound significantly to chitin. A causal relationship was observed between chitin binding activity and antifungal activity. Tachystatins caused morphological changes against a budding yeast, and tachystatin C had a strong cell lysis activity. The septum between mother cell and bud, a chitin-rich region, was stained by fluorescence-labeled tachystatin C, suggesting that the primary recognizing substance on the cell wall is chitin. As horseshoe crab is a close relative of the spider, tachystatins and spider neurotoxins may have evolved from a common ancestral peptide, with adaptive functions.

UR - http://www.scopus.com/inward/record.url?scp=0033543620&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033543620&partnerID=8YFLogxK

U2 - 10.1074/jbc.274.37.26172

DO - 10.1074/jbc.274.37.26172

M3 - Article

C2 - 10473569

AN - SCOPUS:0033543620

VL - 274

SP - 26172

EP - 26178

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 37

ER -