Human γδ T-cell receptor-positive cell-mediated inhibition of erythropoiesis in vitro in a patient with type I autoimmune polyglandular syndrome and pure red blood cell aplasia

T. Hara, Y. Mizuno, M. Nagata, Y. Okabe, S. Taniguchi, M. Harada, Y. Niho, K. Oshimi, S. Ohga, Y. Yoshikai, K. Normoto, K. Yumura, K. Kawa-Ha, K. Ueda

研究成果: Contribution to journalArticle査読

52 被引用数 (Scopus)

抄録

The γδ T-cell receptor-positive (γδTCR+) lymphocytes were markedly expanded up to 68% of peripheral blood lymphocytes in a case with type I autoimmune polyglandular syndrome and pure red blood cell aplasia (PRCA). The γδTCR+ cells showed CD4 negative, 16% dim-CD8 positive and 10% to 46% human leukocyte antigen-D-related (HLA-DR) positive, and exhibited no monoclonality as assessed by the patterns of TCR gene rearrangements. Functional studies revealed that the proliferative responses of the patient's peripheral blood mononuclear cells (PBMC) were severely depressed to candida antigen, alloantigens, and autoantigens (non-T cells). The γδTCR+ cells had no suppressive effect on the proliferative response of the αβTCR+ cells to candida. The patient's PBMC, isolated γδTCR+ cells but not αβTCR+ cells, exhibited non-major histocompatibility complex (MHC)-restricted cytotoxicity. Furthermore, the patient's PBMC and isolated γδTCR+ cells inhibited burst-forming units-erythroid (BFU-E), but not colony-forming units/granulocyte-macrophage (CFU-GM). Supernatants derived from the patient's T cells similarly inhibited BFU-E but not CFU-GM. The clinical course of the patient also showed a close correlation between the decreased number of total lymphocyte counts, especially HLA-DR + γδTCR+ cell counts, and recovery from PRCA. These observations suggest that the γδTCR+ cells might be functional in vivo and involved in the pathogenesis of PRCA in this patient.

本文言語英語
ページ(範囲)941-950
ページ数10
ジャーナルBlood
75
4
DOI
出版ステータス出版済み - 1990

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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