Human aldolase b: Liver-specific properties of the isozyme depend on type b isozyme group-specific sequences

Takahiro Kusakabe, Kiyohisa Motoki, Yasushi Sugimoto, Yozo Takasaki, Katsuji Hori

研究成果: ジャーナルへの寄稿記事

16 引用 (Scopus)

抄録

A series of chimeric enzymes between two human aldolases A, B or C were constructed to identify the molecular regions responsible for isozyme-specific functions. Chimeras constructed between aldolases A and B were AB34 (an AB chimera connected at position 34), ABA34-306 and ABA212-306 (the ABA chimeras). Those between aldolases B and C are BC243, BC263 and BC306 (the BC chimeras connected at positions as indicated), as well as CB55, CB243, CB263 and CB306 (the CB chimeras connected at positions as indicated), CBC55-263 (a CBC chimera), and BCB55-193, BCB55-306, BCB79-193 and BCB79-306 (the BCB chimeric enzymes). Through the analysis of the properties of these chimeras, it was found that for aldolase B, isozyme B group-specific sequences (IGSs)-l and-4 were required for exerting type B-specific functions, while the IGSs-2 and -3 enhanced, in collaboration with the IGS-1, the catalytic activity of aldolase B. In addition, the α/β-barrel and the restricted stretches, which were not specified but occupied two distinct regions spanning the amino acid positions 108-137 (designated connector 1) and 243-306 (designated connector 2), were found to be indispensable for showing full catalytic activity of aldolase B.

元の言語英語
ページ(範囲)1387-1393
ページ数7
ジャーナルProtein Engineering, Design and Selection
7
発行部数11
DOI
出版物ステータス出版済み - 11 1 1994
外部発表Yes

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Fructose-Bisphosphate Aldolase
Liver
Isoenzymes
Catalyst activity
Enzymes
Amino acids
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Molecular Biology

これを引用

Human aldolase b : Liver-specific properties of the isozyme depend on type b isozyme group-specific sequences. / Kusakabe, Takahiro; Motoki, Kiyohisa; Sugimoto, Yasushi; Takasaki, Yozo; Hori, Katsuji.

:: Protein Engineering, Design and Selection, 巻 7, 番号 11, 01.11.1994, p. 1387-1393.

研究成果: ジャーナルへの寄稿記事

Kusakabe, Takahiro ; Motoki, Kiyohisa ; Sugimoto, Yasushi ; Takasaki, Yozo ; Hori, Katsuji. / Human aldolase b : Liver-specific properties of the isozyme depend on type b isozyme group-specific sequences. :: Protein Engineering, Design and Selection. 1994 ; 巻 7, 番号 11. pp. 1387-1393.
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abstract = "A series of chimeric enzymes between two human aldolases A, B or C were constructed to identify the molecular regions responsible for isozyme-specific functions. Chimeras constructed between aldolases A and B were AB34 (an AB chimera connected at position 34), ABA34-306 and ABA212-306 (the ABA chimeras). Those between aldolases B and C are BC243, BC263 and BC306 (the BC chimeras connected at positions as indicated), as well as CB55, CB243, CB263 and CB306 (the CB chimeras connected at positions as indicated), CBC55-263 (a CBC chimera), and BCB55-193, BCB55-306, BCB79-193 and BCB79-306 (the BCB chimeric enzymes). Through the analysis of the properties of these chimeras, it was found that for aldolase B, isozyme B group-specific sequences (IGSs)-l and-4 were required for exerting type B-specific functions, while the IGSs-2 and -3 enhanced, in collaboration with the IGS-1, the catalytic activity of aldolase B. In addition, the α/β-barrel and the restricted stretches, which were not specified but occupied two distinct regions spanning the amino acid positions 108-137 (designated connector 1) and 243-306 (designated connector 2), were found to be indispensable for showing full catalytic activity of aldolase B.",
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