Human aldolase c: Characterization of the recombinant enzyme expressed in Escherichia coli

Takahiro Kusakabe, Kiyohisa Motoki, Katsuji Hori

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

To study the structure/function relationship and enzymatic properties of human aldolase C, we have constructed an Escherichia coli expression plasmid, pHAC11, for the isozyme. E. coli cells carrying this plasmid produced enzymatically active human aldolase C. The kcat and Km values for fructose-l, 6-bisphosphate (Fru-1, 6-P2) and fructose-1-phosphate (Fru-1-P) of the recombinant enzyme were found to be similar to those of authentic aldolase C from human brain. The Fru-1, 6-P2/Fru-1-P activity ratio of the recombinant enzyme is approximately 13.5, which is comparable to that of the recombinant rat aldolase C, but is slightly higher than those of rat brain and hepatoma aldolases C. The substitution of Ser for the carboxyl-terminal Tyr (Tyr-363) of the recombinant enzyme caused a marked decrease in that of Fru-1, 6-P2, with little change in that of Fru-1-P. The activity ratio changed from 13.5 for the normal enzyme to 3.8 for the engineered enzyme. Human aldolase C was found to form tetrameric hybrids with aldolase B in vivo when these enzymes were coexpressed in E. coli cells.

元の言語英語
ページ(範囲)1172-1177
ページ数6
ジャーナルJournal of biochemistry
115
発行部数6
DOI
出版物ステータス出版済み - 1 1 1994
外部発表Yes

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Fructose-Bisphosphate Aldolase
Escherichia coli
Enzymes
Rats
Brain
Plasmids
Fructose
Isoenzymes
Hepatocellular Carcinoma
Substitution reactions

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

Human aldolase c : Characterization of the recombinant enzyme expressed in Escherichia coli. / Kusakabe, Takahiro; Motoki, Kiyohisa; Hori, Katsuji.

:: Journal of biochemistry, 巻 115, 番号 6, 01.01.1994, p. 1172-1177.

研究成果: ジャーナルへの寄稿記事

Kusakabe, Takahiro ; Motoki, Kiyohisa ; Hori, Katsuji. / Human aldolase c : Characterization of the recombinant enzyme expressed in Escherichia coli. :: Journal of biochemistry. 1994 ; 巻 115, 番号 6. pp. 1172-1177.
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