TY - JOUR
T1 - Human complement factor B
T2 - cDNA cloning, nucleotide sequencing, phenotypic conversion by site-directed mutagenesis and expression
AU - Takahiko, Horiuchi
AU - Sunghee, Kim
AU - Mitsuru, Matsumoto
AU - Ichiro, Watanabe
AU - Shigeru, Fujita
AU - Volanakis, John E.
N1 - Funding Information:
*This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan, by the Mochida Memorial Foun-dation for Medical and Pharmaceutical Research, and by US Public Health Service grants AI21067 and AR03555. The nucleotide sequence data presented in this article have been submitted to GenBank data library under accession number L 15702. /[Author to whom correspondence and reprint requests should be addressed at: Division of Clinical Immunology and Rheumatology, UAB Station, Birmingham, AL 35294, U.S.A.
PY - 1993/12
Y1 - 1993/12
N2 - A full-length cDNA clone, BHL4-1, encoding factor B was isolated from a human liver cDNA library and sequenced in its entirety. It consists of 2388 bp which include a 5'-untranslated region of 40 bp, a single open reading frame, 2292 bp in length, and a 3'-untranslated region of 56 bp followed by a poly-A tail. The deduced amino acid sequence comprises 25 residues of a putative leader peptide and 739 residues of the mature polypeptide chain of the F allele of factor B. We constructed an S allele-like Q7R mutant of BHL4-1 by site-directed mutagenesis. Both the wild-type and mutant factor B cDNA were expressed transiently in a eukaryotic system. The specific hemolytic activities of the two recombinant factor B alleles and of native B were not significantly different from each other.
AB - A full-length cDNA clone, BHL4-1, encoding factor B was isolated from a human liver cDNA library and sequenced in its entirety. It consists of 2388 bp which include a 5'-untranslated region of 40 bp, a single open reading frame, 2292 bp in length, and a 3'-untranslated region of 56 bp followed by a poly-A tail. The deduced amino acid sequence comprises 25 residues of a putative leader peptide and 739 residues of the mature polypeptide chain of the F allele of factor B. We constructed an S allele-like Q7R mutant of BHL4-1 by site-directed mutagenesis. Both the wild-type and mutant factor B cDNA were expressed transiently in a eukaryotic system. The specific hemolytic activities of the two recombinant factor B alleles and of native B were not significantly different from each other.
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U2 - 10.1016/0161-5890(93)90450-P
DO - 10.1016/0161-5890(93)90450-P
M3 - Article
C2 - 8247029
AN - SCOPUS:0027139795
VL - 30
SP - 1587
EP - 1592
JO - Immunochemistry
JF - Immunochemistry
SN - 0161-5890
IS - 17
ER -