Human cord blood-derived cells generate insulin-producing cells in vivo

Shuro Yoshida, Fumihiko Ishikawa, Noriaki Kawano, Kazuya Shimoda, Seiho Nagafuchi, Shinji Shimoda, Masaki Yasukawa, Takaaki Kanemaru, Hiromi Ishibashi, Leonard D. Shultz, Mine Harada

研究成果: Contribution to journalArticle査読

66 被引用数 (Scopus)

抄録

Here we report the capacity of human cord blood (CB)-derived cells to generate insulin-producing cells. To investigate in vivo capacity of human CB-derived cells, T cell-depleted mononuclear cells were intravenously transplanted into nonobese diabetic/severe combined immunodeficient/ β2-microglobulinnull mice within 48 hours of birth. At 1-2 months post-transplantation, immunofluorescence staining for insulin and fluorescence in situ hybridization (FISH) analysis using a human chromosome probe indicated that human CB-derived cells generated insulin-producing cells at a frequency of 0.65% ± 0.64% in xenogeneic hosts. Reverse transcription-polymerase chain reaction analysis confirmed the transcription of human insulin in the pancreatic tissue of the recipient mice. To clarify the mechanism underlying CB-derived insulin-producing cells, double FISH analysis using species-specific probes was performed. Almost equal proportions of human chromosome+ murine chromosome- insulin+ cells and human chromosome+ murine chromosome+ insulin + cells were present in recipient pancreatic islets. Taken together, human CB contains progenitor cells, Avhich can generate insulin-producing cells in recipient pancreatic tissues across a xenogeneic histocompatibility barrier by fusion-dependent and -independent mechanisms.

本文言語英語
ページ(範囲)1409-1416
ページ数8
ジャーナルSTEM CELLS
23
9
DOI
出版ステータス出版済み - 10 2005

All Science Journal Classification (ASJC) codes

  • 分子医療
  • 発生生物学
  • 細胞生物学

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