Human cord blood-derived cells generate insulin-producing cells in vivo

Shuro Yoshida, Fumihiko Ishikawa, Noriaki Kawano, Kazuya Shimoda, Seiho Nagafuchi, Shinji Shimoda, Masaki Yasukawa, Takaaki Kanemaru, Hiromi Ishibashi, Leonard D. Shultz, Mine Harada

研究成果: ジャーナルへの寄稿記事

66 引用 (Scopus)

抄録

Here we report the capacity of human cord blood (CB)-derived cells to generate insulin-producing cells. To investigate in vivo capacity of human CB-derived cells, T cell-depleted mononuclear cells were intravenously transplanted into nonobese diabetic/severe combined immunodeficient/ β2-microglobulinnull mice within 48 hours of birth. At 1-2 months post-transplantation, immunofluorescence staining for insulin and fluorescence in situ hybridization (FISH) analysis using a human chromosome probe indicated that human CB-derived cells generated insulin-producing cells at a frequency of 0.65% ± 0.64% in xenogeneic hosts. Reverse transcription-polymerase chain reaction analysis confirmed the transcription of human insulin in the pancreatic tissue of the recipient mice. To clarify the mechanism underlying CB-derived insulin-producing cells, double FISH analysis using species-specific probes was performed. Almost equal proportions of human chromosome+ murine chromosome- insulin+ cells and human chromosome+ murine chromosome+ insulin + cells were present in recipient pancreatic islets. Taken together, human CB contains progenitor cells, Avhich can generate insulin-producing cells in recipient pancreatic tissues across a xenogeneic histocompatibility barrier by fusion-dependent and -independent mechanisms.

元の言語英語
ページ(範囲)1409-1416
ページ数8
ジャーナルSTEM CELLS
23
発行部数9
DOI
出版物ステータス出版済み - 10 1 2005

Fingerprint

Fetal Blood
Blood Cells
Insulin
Human Chromosomes
Fluorescence In Situ Hybridization
Chromosomes
Histocompatibility
Islets of Langerhans
Reverse Transcription
Fluorescent Antibody Technique
Stem Cells
Transplantation
Parturition
Staining and Labeling
T-Lymphocytes
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

これを引用

Yoshida, S., Ishikawa, F., Kawano, N., Shimoda, K., Nagafuchi, S., Shimoda, S., ... Harada, M. (2005). Human cord blood-derived cells generate insulin-producing cells in vivo. STEM CELLS, 23(9), 1409-1416. https://doi.org/10.1634/stemcells.2005-0079

Human cord blood-derived cells generate insulin-producing cells in vivo. / Yoshida, Shuro; Ishikawa, Fumihiko; Kawano, Noriaki; Shimoda, Kazuya; Nagafuchi, Seiho; Shimoda, Shinji; Yasukawa, Masaki; Kanemaru, Takaaki; Ishibashi, Hiromi; Shultz, Leonard D.; Harada, Mine.

:: STEM CELLS, 巻 23, 番号 9, 01.10.2005, p. 1409-1416.

研究成果: ジャーナルへの寄稿記事

Yoshida, S, Ishikawa, F, Kawano, N, Shimoda, K, Nagafuchi, S, Shimoda, S, Yasukawa, M, Kanemaru, T, Ishibashi, H, Shultz, LD & Harada, M 2005, 'Human cord blood-derived cells generate insulin-producing cells in vivo', STEM CELLS, 巻. 23, 番号 9, pp. 1409-1416. https://doi.org/10.1634/stemcells.2005-0079
Yoshida S, Ishikawa F, Kawano N, Shimoda K, Nagafuchi S, Shimoda S その他. Human cord blood-derived cells generate insulin-producing cells in vivo. STEM CELLS. 2005 10 1;23(9):1409-1416. https://doi.org/10.1634/stemcells.2005-0079
Yoshida, Shuro ; Ishikawa, Fumihiko ; Kawano, Noriaki ; Shimoda, Kazuya ; Nagafuchi, Seiho ; Shimoda, Shinji ; Yasukawa, Masaki ; Kanemaru, Takaaki ; Ishibashi, Hiromi ; Shultz, Leonard D. ; Harada, Mine. / Human cord blood-derived cells generate insulin-producing cells in vivo. :: STEM CELLS. 2005 ; 巻 23, 番号 9. pp. 1409-1416.
@article{6282bfce20484c389c9fc01390ffda4e,
title = "Human cord blood-derived cells generate insulin-producing cells in vivo",
abstract = "Here we report the capacity of human cord blood (CB)-derived cells to generate insulin-producing cells. To investigate in vivo capacity of human CB-derived cells, T cell-depleted mononuclear cells were intravenously transplanted into nonobese diabetic/severe combined immunodeficient/ β2-microglobulinnull mice within 48 hours of birth. At 1-2 months post-transplantation, immunofluorescence staining for insulin and fluorescence in situ hybridization (FISH) analysis using a human chromosome probe indicated that human CB-derived cells generated insulin-producing cells at a frequency of 0.65{\%} ± 0.64{\%} in xenogeneic hosts. Reverse transcription-polymerase chain reaction analysis confirmed the transcription of human insulin in the pancreatic tissue of the recipient mice. To clarify the mechanism underlying CB-derived insulin-producing cells, double FISH analysis using species-specific probes was performed. Almost equal proportions of human chromosome+ murine chromosome- insulin+ cells and human chromosome+ murine chromosome+ insulin + cells were present in recipient pancreatic islets. Taken together, human CB contains progenitor cells, Avhich can generate insulin-producing cells in recipient pancreatic tissues across a xenogeneic histocompatibility barrier by fusion-dependent and -independent mechanisms.",
author = "Shuro Yoshida and Fumihiko Ishikawa and Noriaki Kawano and Kazuya Shimoda and Seiho Nagafuchi and Shinji Shimoda and Masaki Yasukawa and Takaaki Kanemaru and Hiromi Ishibashi and Shultz, {Leonard D.} and Mine Harada",
year = "2005",
month = "10",
day = "1",
doi = "10.1634/stemcells.2005-0079",
language = "English",
volume = "23",
pages = "1409--1416",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "AlphaMed Press",
number = "9",

}

TY - JOUR

T1 - Human cord blood-derived cells generate insulin-producing cells in vivo

AU - Yoshida, Shuro

AU - Ishikawa, Fumihiko

AU - Kawano, Noriaki

AU - Shimoda, Kazuya

AU - Nagafuchi, Seiho

AU - Shimoda, Shinji

AU - Yasukawa, Masaki

AU - Kanemaru, Takaaki

AU - Ishibashi, Hiromi

AU - Shultz, Leonard D.

AU - Harada, Mine

PY - 2005/10/1

Y1 - 2005/10/1

N2 - Here we report the capacity of human cord blood (CB)-derived cells to generate insulin-producing cells. To investigate in vivo capacity of human CB-derived cells, T cell-depleted mononuclear cells were intravenously transplanted into nonobese diabetic/severe combined immunodeficient/ β2-microglobulinnull mice within 48 hours of birth. At 1-2 months post-transplantation, immunofluorescence staining for insulin and fluorescence in situ hybridization (FISH) analysis using a human chromosome probe indicated that human CB-derived cells generated insulin-producing cells at a frequency of 0.65% ± 0.64% in xenogeneic hosts. Reverse transcription-polymerase chain reaction analysis confirmed the transcription of human insulin in the pancreatic tissue of the recipient mice. To clarify the mechanism underlying CB-derived insulin-producing cells, double FISH analysis using species-specific probes was performed. Almost equal proportions of human chromosome+ murine chromosome- insulin+ cells and human chromosome+ murine chromosome+ insulin + cells were present in recipient pancreatic islets. Taken together, human CB contains progenitor cells, Avhich can generate insulin-producing cells in recipient pancreatic tissues across a xenogeneic histocompatibility barrier by fusion-dependent and -independent mechanisms.

AB - Here we report the capacity of human cord blood (CB)-derived cells to generate insulin-producing cells. To investigate in vivo capacity of human CB-derived cells, T cell-depleted mononuclear cells were intravenously transplanted into nonobese diabetic/severe combined immunodeficient/ β2-microglobulinnull mice within 48 hours of birth. At 1-2 months post-transplantation, immunofluorescence staining for insulin and fluorescence in situ hybridization (FISH) analysis using a human chromosome probe indicated that human CB-derived cells generated insulin-producing cells at a frequency of 0.65% ± 0.64% in xenogeneic hosts. Reverse transcription-polymerase chain reaction analysis confirmed the transcription of human insulin in the pancreatic tissue of the recipient mice. To clarify the mechanism underlying CB-derived insulin-producing cells, double FISH analysis using species-specific probes was performed. Almost equal proportions of human chromosome+ murine chromosome- insulin+ cells and human chromosome+ murine chromosome+ insulin + cells were present in recipient pancreatic islets. Taken together, human CB contains progenitor cells, Avhich can generate insulin-producing cells in recipient pancreatic tissues across a xenogeneic histocompatibility barrier by fusion-dependent and -independent mechanisms.

UR - http://www.scopus.com/inward/record.url?scp=27144462791&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27144462791&partnerID=8YFLogxK

U2 - 10.1634/stemcells.2005-0079

DO - 10.1634/stemcells.2005-0079

M3 - Article

C2 - 16210412

AN - SCOPUS:27144462791

VL - 23

SP - 1409

EP - 1416

JO - Stem Cells

JF - Stem Cells

SN - 1066-5099

IS - 9

ER -