Human foreskin fibroblasts exert immunomodulatory properties by a different mechanism to bone marrow stromal/stem cells

Naohisa Wada, Peter Mark Bartold, Stan Gronthos

研究成果: ジャーナルへの寄稿記事

41 引用 (Scopus)

抄録

Human bone marrow mesenchymal stromal/stem cells (BMSCs) have been reported to possess immunomodulatory functions with the capacity to suppress immune reactions partly mediated by immunosuppressive factors, indoleamine 2,3-dioxygenase and nitric oxide, and suggested to be potentially applicable for therapeutic use. More recently, other fibroblast-like cells have been reported to possess similar properties. Here we demonstrate that human foreskin fibroblasts (HFFs) express an MSC-like immunophenotype and possess immunosuppressive properties similar to BMSCs but lack the capacity for osteogenic and adipogenic differentiation. HFFs suppressed human peripheral blood mononuclear cells (PBMC) proliferation stimulated with mitogen or in an allogeneic mixed lymphocyte reaction comparable to BMSCs. However, HFFs showed undetectable levels of indoleamine 2,3-dioxygenase and inducible nitric oxide synthase expression, in contrast to BMSCs when cocultured with activated PBMCs. To identify HFF specific immunosuppressive factors, we performed array profiling of common cytokines expressed by HFFs and BMSCs alone or when cocultured with activated PBMCs. Real-time polymerase chain reaction analysis confirmed that multiple factors were upregulated in HFFs cocultured with activated PBMCs compared with HFFs alone or BMSCs cultured under the same conditions. Functional assays identified interferon-α as the major immunosuppressive mediator expressed by HFFs. These results suggest that the HFFs possess immunosuppressive properties, which are mediated by alternate mechanisms to that reported for BMSCs.

元の言語英語
ページ(範囲)647-659
ページ数13
ジャーナルStem cells and development
20
発行部数4
DOI
出版物ステータス出版済み - 4 1 2011
外部発表Yes

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Foreskin
Mesenchymal Stromal Cells
Stem Cells
Fibroblasts
Immunosuppressive Agents
Indoleamine-Pyrrole 2,3,-Dioxygenase

All Science Journal Classification (ASJC) codes

  • Hematology
  • Developmental Biology
  • Cell Biology

これを引用

Human foreskin fibroblasts exert immunomodulatory properties by a different mechanism to bone marrow stromal/stem cells. / Wada, Naohisa; Bartold, Peter Mark; Gronthos, Stan.

:: Stem cells and development, 巻 20, 番号 4, 01.04.2011, p. 647-659.

研究成果: ジャーナルへの寄稿記事

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