Human umbilical cord-derived mesenchymal stromal cells differentiate into functional schwann cells that sustain peripheral nerve regeneration

Dai Matsuse, Masaaki Kitada, Misaki Kohama, Kouki Nishikawa, Hideki Makinoshima, Shohei Wakao, Yoshinori Fujiyoshi, Toshio Heike, Tatsutoshi Nakahata, Hidenori Akutsu, Akihiro Umezawa, Hideo Harigae, Jun-Ichi Kira, Mari Dezawa

研究成果: ジャーナルへの寄稿記事

68 引用 (Scopus)

抄録

Human umbilical cord-derived mesenchymal stromal cells (UC-MSCs) that are available from cell banks can be induced to differentiate into various cell types, thereby making them practical potential sources for cell-based therapies. In injured peripheral nerves, Schwann cells (SCs) contribute to functional recovery by supporting axonal regeneration and myelin reconstruction. Here, we first demonstrate a system toinduce UC-MSCs to differentiate into cells with SC properties (UC-SCs) by treatment with β-mercaptoethanol followed by retinoic acid and a set of specific cytokines. The UC-SCs are morphologically similar to SCs and express SC markers, including P0, as assessed by immunocytochemistry and reverse transcription polymerase chain reaction. Transplantation of UC-SCs into transected sciatic nerves in adult rats enhanced nerve regeneration. The effectiveness of UC-SCs for axonal regeneration was comparable to that of authentic human SCs based on histological criteria and functional recovery. Immunohistochemistry and immunoelectron microscopy also demonstrated myelination of regenerated axons by UC-SCs. These findings indicate that cells with SC properties and with the ability to support axonal regeneration and reconstruct myelin can be successfully induced from UC-MSCs to promote functional recovery after peripheral nerve injury. This system may be applicable for the development of cell-based therapies.

元の言語英語
ページ(範囲)973-985
ページ数13
ジャーナルJournal of Neuropathology and Experimental Neurology
69
発行部数9
DOI
出版物ステータス出版済み - 1 1 2010

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Nerve Regeneration
Umbilical Cord
Schwann Cells
Mesenchymal Stromal Cells
Peripheral Nerves
Regeneration
Myelin Sheath
Cell- and Tissue-Based Therapy
Immunohistochemistry
Peripheral Nerve Injuries
Immunoelectron Microscopy
Mercaptoethanol
Sciatic Nerve
Tretinoin
Reverse Transcription
Axons
Transplantation
Cytokines

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

これを引用

Human umbilical cord-derived mesenchymal stromal cells differentiate into functional schwann cells that sustain peripheral nerve regeneration. / Matsuse, Dai; Kitada, Masaaki; Kohama, Misaki; Nishikawa, Kouki; Makinoshima, Hideki; Wakao, Shohei; Fujiyoshi, Yoshinori; Heike, Toshio; Nakahata, Tatsutoshi; Akutsu, Hidenori; Umezawa, Akihiro; Harigae, Hideo; Kira, Jun-Ichi; Dezawa, Mari.

:: Journal of Neuropathology and Experimental Neurology, 巻 69, 番号 9, 01.01.2010, p. 973-985.

研究成果: ジャーナルへの寄稿記事

Matsuse, D, Kitada, M, Kohama, M, Nishikawa, K, Makinoshima, H, Wakao, S, Fujiyoshi, Y, Heike, T, Nakahata, T, Akutsu, H, Umezawa, A, Harigae, H, Kira, J-I & Dezawa, M 2010, 'Human umbilical cord-derived mesenchymal stromal cells differentiate into functional schwann cells that sustain peripheral nerve regeneration', Journal of Neuropathology and Experimental Neurology, 巻. 69, 番号 9, pp. 973-985. https://doi.org/10.1097/NEN.0b013e3181eff6dc
Matsuse, Dai ; Kitada, Masaaki ; Kohama, Misaki ; Nishikawa, Kouki ; Makinoshima, Hideki ; Wakao, Shohei ; Fujiyoshi, Yoshinori ; Heike, Toshio ; Nakahata, Tatsutoshi ; Akutsu, Hidenori ; Umezawa, Akihiro ; Harigae, Hideo ; Kira, Jun-Ichi ; Dezawa, Mari. / Human umbilical cord-derived mesenchymal stromal cells differentiate into functional schwann cells that sustain peripheral nerve regeneration. :: Journal of Neuropathology and Experimental Neurology. 2010 ; 巻 69, 番号 9. pp. 973-985.
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abstract = "Human umbilical cord-derived mesenchymal stromal cells (UC-MSCs) that are available from cell banks can be induced to differentiate into various cell types, thereby making them practical potential sources for cell-based therapies. In injured peripheral nerves, Schwann cells (SCs) contribute to functional recovery by supporting axonal regeneration and myelin reconstruction. Here, we first demonstrate a system toinduce UC-MSCs to differentiate into cells with SC properties (UC-SCs) by treatment with β-mercaptoethanol followed by retinoic acid and a set of specific cytokines. The UC-SCs are morphologically similar to SCs and express SC markers, including P0, as assessed by immunocytochemistry and reverse transcription polymerase chain reaction. Transplantation of UC-SCs into transected sciatic nerves in adult rats enhanced nerve regeneration. The effectiveness of UC-SCs for axonal regeneration was comparable to that of authentic human SCs based on histological criteria and functional recovery. Immunohistochemistry and immunoelectron microscopy also demonstrated myelination of regenerated axons by UC-SCs. These findings indicate that cells with SC properties and with the ability to support axonal regeneration and reconstruct myelin can be successfully induced from UC-MSCs to promote functional recovery after peripheral nerve injury. This system may be applicable for the development of cell-based therapies.",
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