Human Werner helicase interacting protein 1 (WRNIP1) functions as novel modulator for DNA polymerase δ

Toshiki Tsurimoto, Ayako Shinozaki, Masaki Yano, Masayuki Seki, Takemi Enomoto

研究成果: ジャーナルへの寄稿記事

41 引用 (Scopus)

抄録

Human WRNIP1, a Werner DNA helicase interacting protein 1, was expressed in insect cells and E. coli. The purified protein behaved as a homo-oligomeric complex with a native molecular mass indicative of an octamer, and the complex copurified with an ATPase activity that was stimulated by double-stranded DNA ends. As suggested by genetic studies of budding yeast WRNIP1/Mgs1, the purified human WRNIP1 complex interacted physically with human DNA polymerase δ (pol δ), stimulating its DNA synthesis activity more than fivefold in the presence or absence of proliferating cell nuclear antigen. Analysis of reaction products demonstrated the stimulation to be partly due to an increased processivity of pol δ but more importantly to an increase in its initiation frequency. Addition of ATP to reactions partially suppressed stimulation by WRNIP1. Furthermore, a mutant WRNIP1 lacking ATPase activity could stimulate pol δ normally but was insensitive to suppression by ATP. These results indicate that WRNIP1 functions as a modulator for initiation or restart events during pol δ-mediated DNA synthesis and that its ATPase activity is utilized to sense DNA ends and to regulate the extent of stimulation.

元の言語英語
ページ(範囲)13-22
ページ数10
ジャーナルGenes to Cells
10
発行部数1
DOI
出版物ステータス出版済み - 1 2005

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DNA-Directed DNA Polymerase
Adenosine Triphosphatases
Proteins
DNA
Adenosine Triphosphate
DNA Helicases
Saccharomycetales
Proliferating Cell Nuclear Antigen
Insects
human WRNIP1 protein
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

これを引用

Human Werner helicase interacting protein 1 (WRNIP1) functions as novel modulator for DNA polymerase δ. / Tsurimoto, Toshiki; Shinozaki, Ayako; Yano, Masaki; Seki, Masayuki; Enomoto, Takemi.

:: Genes to Cells, 巻 10, 番号 1, 01.2005, p. 13-22.

研究成果: ジャーナルへの寄稿記事

Tsurimoto, Toshiki ; Shinozaki, Ayako ; Yano, Masaki ; Seki, Masayuki ; Enomoto, Takemi. / Human Werner helicase interacting protein 1 (WRNIP1) functions as novel modulator for DNA polymerase δ. :: Genes to Cells. 2005 ; 巻 10, 番号 1. pp. 13-22.
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