Hydrazine radical formation catalyzed by rat microsomal NADPH-cytochrome P-450 reductase

Atsuko Noda, Hiroshi Noda, Ayako Misaka, Hideki Sumimoto, Kiyoshi Tatsumi

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

Using NADPH-cytochrome P-450 reductase purified from rat liver microsomes, the oxidation of hydrazine to its radical was proved to proceed smoothly. The catalytic effect of NADPH-cytochrome P-450 on the radical formation in the hepatic microsomes obtained from phenobarbital-pretreated rats was also supported by the fact that Hz radical formation was stimulated by flavin adenin dinucleotide or methyl viologen and markedly inhibited by superoxide dismutase, however, carbon monoxide showed no effect. Expectedly, anti-NADPH-cytochrome P-450 IgG decreased the radical formation. The present study provides the first evidence for the NADPH-cytochrome P-450 reductase catalyzed oxidation of hydrazine to its radical in the presence of O2 and NADPH.

元の言語英語
ページ(範囲)256-260
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
153
発行部数1
DOI
出版物ステータス出版済み - 5 31 1988

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hydrazine
NADPH-Ferrihemoprotein Reductase
NADP
Rats
Cytochrome P-450 Enzyme System
Oxidation
Paraquat
Liver Microsomes
Carbon Monoxide
Phenobarbital
Microsomes
Liver
Superoxide Dismutase
Immunoglobulin G

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

これを引用

Hydrazine radical formation catalyzed by rat microsomal NADPH-cytochrome P-450 reductase. / Noda, Atsuko; Noda, Hiroshi; Misaka, Ayako; Sumimoto, Hideki; Tatsumi, Kiyoshi.

:: Biochemical and Biophysical Research Communications, 巻 153, 番号 1, 31.05.1988, p. 256-260.

研究成果: ジャーナルへの寄稿記事

Noda, Atsuko ; Noda, Hiroshi ; Misaka, Ayako ; Sumimoto, Hideki ; Tatsumi, Kiyoshi. / Hydrazine radical formation catalyzed by rat microsomal NADPH-cytochrome P-450 reductase. :: Biochemical and Biophysical Research Communications. 1988 ; 巻 153, 番号 1. pp. 256-260.
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