Hydrodynamics of cryogelation

Keiichi Miyamoto, Yoshiyuki Shimonishi, Masayuki Tokita, Takashi Komai

研究成果: ジャーナルへの寄稿学術誌査読

3 被引用数 (Scopus)

抄録

Cryogel, prevalent in the plasma of rheumatoid arthritis patients, is a plasma fibronectin (pFN)-extra domain A containing FN [EDA(+)FN]-fibrinogen (Fbg) aggregate formed by the addition of heparin (Hep) at low temperature. Although EDA(+)FN is not usually present in normal plasma, its prevalence in rheumatic patients induces cryogelation. In this study, we determined the hydrodynamic radius (Rh) ratio (Rh/Rh30) of the cryogel component by dynamic light scattering in vitro. Rh/Rh30 was normalized to Rh at 30 °C (Rh30) at several temperatures. The Rh/Rh30 of Fbg was found to increase only by self-aggregation, whereas the Rh/Rh30 of FNs does not increase in response to temperature changes. The Rh/Rh30 of the Fbg/FN aggregate is increased by the addition of Hep, and the Rh/Rh30 (12.5) of the Hep-induced EDA(+)FN/Fbg aggregate is greater than that (2.5) of the pFN/Fbg aggregate. These results suggest that cryogelation requires Fbg self-aggregation and the interaction between EDA(+)FN and Hep.

本文言語英語
ページ(範囲)219-223
ページ数5
ジャーナルInternational Journal of Biological Macromolecules
29
4-5
DOI
出版ステータス出版済み - 12月 10 2001
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 生化学
  • 分子生物学
  • 経済学、計量経済学
  • エネルギー(全般)

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