Hydrogen peroxide induced responses of cat tracheal smooth muscle cells

V. Bauer, M. Oike, H. Tanaka, R. Inoue, Y. Ito

研究成果: ジャーナルへの寄稿記事

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1. The effects of hydrogen peroxide (H2O2) (10-6-10-3 M) on membrane potential, membrane currents, intracellular calcium concentration, resting muscle tone and contractions elicited by electrical field stimulation (EFS) and carbachol were examined in cat tracheal strips and isolated smooth muscle cells. 2. H2O2 (10-4 and 10-5 M) enhanced the amplitude of contractions and excitatory junction potentials (e.j.p.) evoked by EFS without changing muscle tone and resting membrane potential of the tracheal smooth muscle, and enhanced the contraction induced by carbachol (10-8 M). At an increased concentration (10-3 M), H2O2 elevated resting muscle tone and marginally hyperpolarized the membrane in the majority of the cells. 3. In 51 out of 56 cells examined, H2O2 (10-6-10-3 M) elicited an outward current at a holding potential of -40 mV and enhanced the frequency of the spontaneous transient outward current (STOC). In 20 cells the outward current was preceded by a small inward current. In the other cells, H2O2 elicited only an inward current or did not affect the background current. 4. In Ca2+ free solution the action of H2O2 on the resting muscle tone, STOCs, background current and on the current induced by ramp depolarization was significantly reduced. 5. H2O2 (10-4 M) increased the intracellular ionized calcium concentration both in the absence and presence of external Ca2+. However, the effect developed faster and was of a higher amplitude in the presence of external Ca2+. 6. These results suggest that H2O2 increases intracellular Ca2+, with a subsequent augmentation of stimulation-evoked contractions, and enhances Ca2+ and voltage-sensitive potassium conductance.

元の言語英語
ページ(範囲)867-874
ページ数8
ジャーナルBritish Journal of Pharmacology
121
発行部数5
DOI
出版物ステータス出版済み - 7 15 1997

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All Science Journal Classification (ASJC) codes

  • Pharmacology

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