Identification and characterization of two novel classes of small RNAs in the mouse germline: Retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes

Toshiaki Watanabe, Atsushi Takeda, Tomoyuki Tsukiyama, Kazuyuki Mise, Tetsuro Okuno, Hiroyuki Sasaki, Naojiro Minami, Hiroshi Imai

研究成果: ジャーナルへの寄稿学術誌査読

461 被引用数 (Scopus)

抄録

Small RNAs ranging in size between 18 and 30 nucleotides (nt) are found in many organisms including yeasts, plants, and animals. Small RNAs are involved in the regulation of gene expression through translational repression, mRNA degradation, and chromatin modification. In mammals, microRNAs (miRNAs) are the only small RNAs that have been well characterized. Here, we have identified two novel classes of small RNAs in the mouse germline. One class consists of ∼20- to 24-nt small interfering RNAs (siRNAs) from mouse oocytes, which are derived from retroelements including LINE, SINE, and LTR retrotransposons. Addition of retrotransposon-derived sequences to the 3′ untranslated region (UTR) of a reporter mRNA destabilizes the mRNA significantly when injected into full-grown oocytes. These results suggest that retrotransposons are suppressed through the RNAi pathway in mouse oocytes. The other novel class of small RNAs is 26- to 30-nt germline small RNAs (gsRNAs) from testes. gsRNAs are expressed during spermatogenesis in a developmentally regulated manner, are mapped to the genome in clusters, and have strong strand bias. These features are reminiscent of Tetrahymena ∼23- to 24-nt small RNAs and Caenorhabditis elegans X-cluster small RNAs. A conserved novel small RNA pathway may be present in diverse animals.

本文言語英語
ページ(範囲)1732-1743
ページ数12
ジャーナルGenes and Development
20
13
DOI
出版ステータス出版済み - 7月 1 2006
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 遺伝学
  • 発生生物学

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