Identification and expression analysis of minichromosome maintenance proteins in the silkworm, Bombyx mori

Kohji Yamamoto, Yoichi Aso, Hiroshi Fujii

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

The minichromosome maintenance protein (MCM) family is involved in the regulatory role of DNA replication in eukaryotic organisms. A cDNA encoding of an MCM of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequenced. The resultant amino acid sequence and phylogenetic analysis revealed high identity to MCM, and specifically to MCM7, of vertebrates and invertebrates. An RT-PCR showed that the bmMCM7 transcript was present in the ovaries, testes, silk glands, and fat bodies of larval silkworms. Expression plasmids were transformed into competent Escherichia coli and overexpressed. This is the first report on the identification of MCM helicase of the silkworm, B. mori.

本文言語英語
論文番号148
ジャーナルJournal of Insect Science
10
DOI
出版ステータス出版済み - 2010

All Science Journal Classification (ASJC) codes

  • 昆虫科学

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