Identification and molecular characterization of a sex chromosome rearrangement causing a soft and pliable (spli) larval body phenotype in the silkworm, bombyx mori

Tsuguru Fujii, Seigo Kuwazaki, Kimiko Yamamoto, Hiroaki Abe, Akio Ohnuma, Susumu Katsuma, Kazuei Mita, Toru Shimada

研究成果: ジャーナルへの寄稿学術誌査読

4 被引用数 (Scopus)

抄録

We carried out genetic and cytogenetic analyses of X-ray-induced deleterious Z chromosomes that result in a soft and pliable (spli) phenotype in the silkworm, Bombyx mori. In a B. mori strain with a spli phenotype, we found the Z chromosome broken between the sch (1-21.5) and od (1-49.6) loci. We also found a chromosomal fragment bearing a fifth-chromosome locus for egg and eye pigmentation fused to a Z chromosome fragment. By means of fluorescence in situ hybridization using bacterial artificial chromosome clones as probes, we confirmed that the fused chromosome is composed of a fragment of chromosome 5 and a fragment of the Z chromosome. Moreover, a predicted gene, GA002017, the Bombyx ortholog of the Drosophila gene acj6 (Bmacj6), was completely deleted by the Z chromosome breakage event. The relationship between Bmacj6 and the spli phenotype is discussed.

本文言語英語
ページ(範囲)45-54
ページ数10
ジャーナルGenome
53
1
DOI
出版ステータス出版済み - 1月 1 2010
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分子生物学
  • 遺伝学

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