Identification of 3-Methylbutanoyl Glycosides in Green Coffea arabica Beans as Causative Determinants for the Quality of Coffee Flavors

Keiko Iwasa, Daiki Setoyama, Hiroaki Shimizu, Harumichi Seta, Yoshinori Fujimura, Daisuke Miura, Hiroyuki Wariishi, Chifumi Nagai, Koichi Nakahara

研究成果: ジャーナルへの寄稿学術誌査読

19 被引用数 (Scopus)

抄録

The quality of coffee green beans is generally evaluated by the sensory cupping test, rather than by chemical compound-based criteria. In this study, we examined the relationship between metabolites and cupping scores for 36 varieties of beans, using a nontargeted LC-MS-based metabolic profiling technique. The cupping score was precisely predicted with the metabolic information measured using LC-MS. Two markers that strongly correlated with high cupping scores were determined to be isomers of 3-methylbutanoyl disaccharides (3MDs; 0.01-0.035 g/kg of beans) by spectroscopic analyses after purification, and one of them was a novel structure. Further, both the 3MDs were determined to be precursors of 3-methylbutanoic acid that enhance the quality of coffee. The applicability of 3MDs as universal quality indicators was validated with another sample set. It was concluded that 3MDs are the causative metabolites determining beverage quality and can be utilized for green bean selection and as key compounds for improving the beverage quality.

本文言語英語
ページ(範囲)3742-3751
ページ数10
ジャーナルJournal of Agricultural and Food Chemistry
63
14
DOI
出版ステータス出版済み - 4月 15 2015

!!!All Science Journal Classification (ASJC) codes

  • 化学 (全般)
  • 農業および生物科学(全般)

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